| γ-亚麻酸在RAW264.7细胞中的抗炎症作用机制（英文） |
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| 引用本文： | 孔秀芹,殷志敏,罗兰.γ-亚麻酸在RAW264.7细胞中的抗炎症作用机制（英文）[J].农业科学与技术,2010,11(6):1-3. |
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| 作者姓名： | 孔秀芹 殷志敏 罗兰 |
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| 作者单位： | 孔秀芹,殷志敏(南京师范大学生命科学学院江苏省分子医学生物技术重点实验室,江苏南京,210046);罗兰(南京大学生命科学学院医药生物技术国家重点实验室,江苏南京,210093) |
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| 摘 要： | 目的]探讨γ-亚麻酸（GLA）对脂多糖（LPS）诱导的RAW264.7细胞产生炎症介质的影响及其机制。方法]采用体外培养的巨噬细胞系RAW264.7细胞,待细胞生长至融合状态后加入不同浓度（0、12.5、25、50μmol/L）的GLA预孵4h,利用100ng/ml的脂多糖（LPS）刺激12h或0.5h,同时设空白对照和LPS对照,利用蛋白印迹法检测诱生型一氧化氮合酶（iNOS）、环加氧酶-2（COX-2）蛋白的表达以及对IκBα、p-JNK/SAPK（Thr183/Tyr185）、p38MAPK、p-p38MAPK（Thr180/Tyr182）、ERK1/2、p-ERK1/2的影响。结果]GLA可以显著抑制LPS诱导的RAW264.7细胞中iNOS和COX-2的蛋白表达（P〈0.05）,且在0～50μmol/LGLA浓度范围内存在剂量依赖关系。GLA可以显著抑制IκBα的降解（P〈0.05）,从而抑制NF-κB的激活。GLA可以显著抑制LPS诱导的JNK1/2以及ERK1/2的磷酸化（P〈0.05）,对p38的磷酸化没有显著的影响。结论]GLA具有很好的消炎功效。抑制JNK1/2以及ERK1/2的磷酸化、抑制NF-κB的激活可能是其发挥生物学效应的重要机制。
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| 关 键 词： | γ-亚麻酸 内毒素 炎症 |
Anti-inflammatory Mechanism of Gamma-linolenic Acid in RAW264.7 Cells |
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| KONG Xiu-qin,YIN Zhi-min,LUO Lan.Anti-inflammatory Mechanism of Gamma-linolenic Acid in RAW264.7 Cells[J].Agricultural Science ＆ Technology,2010,11(6):1-3. |
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| Authors: | KONG Xiu-qin YIN Zhi-min LUO Lan |
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| Institution: | 1.Jiangsu Provinclal Key Laboratory for Molecular and Medical Biotechnology,College of Life Sciences,Nanjing Normal University,Nanjing 210097;2.State Key Laboratory of Pharmaceutical Biotechnology,School of Life Sciences,Nanjing University,Nanjing 210093 |
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| Abstract: | Objective] The aim was to investigate the anti-inflammatory effect and the mechanism of gamma-linolenic acid on lipopolysaccharide-induced RAW264.7 cells.Method] Macrophagic system RAW 264.7 cells were cultured in vitro,when cells grew to fusion state,they were pretreated with 0,12.5,25.0,50.0 μmol/L of GLA for 4 h,and then 100 ng/ml of LPS were added to induce for 12 h or 30 min.Meanwhile,the blank control and LPS control were set.And the expression of iNOS,COX-2 and the effect of GLA on IκBα,p-JNK/SAPK（Thr183/Tyr185）,p38 MAPK,p-p38 MAPK（Thr180/Tyr182）,ERK1/2,p-ERK1/2 were detected by Western blot.Result] GLA significantly inhibited the expression of iNOS and COX-2 in RAW264.7 cells induced by LPS,and in the range of 0-50 μmol/L of GLA,the inhibition effect was concentration-dependent（P0.05）.GLA could significantly inhibited the degradation of IκBα（P0.05）,thereby inhibited the activation of NF-κB.GLA could significantly inhibited the phosphorylation of LPS-induced JNK1/2 and ERK1/2（P0.05）,while it had not significantly effect on the phosphorylation of p38（P0.05）.Conclusion] GLA had excellent anti-inflammation effect.The inhibition of the phosphorylation of JNK1/2,ERK1/2 and the inhibition of activation of NF-κB might be the important mechanism for the educing of its biological effect. |
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| Keywords: | Gamma-linolenic acid Endotoxin Inflammation |
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