| 第二代测序技术在植物遗传研究中的应用 |
| |
| 引用本文： | 罗 纯,张青林,罗正荣.第二代测序技术在植物遗传研究中的应用[J].广东农业科学,2015,42(3):186-192. |
| |
| 作者姓名： | 罗 纯 张青林 罗正荣 |
| |
| 作者单位： | 1. 中国热带农业科学院南亚热带作物研究所/农业部热带果树生物学重点实验室,广东湛江524091;华中农业大学园艺植物生物学教育部重点实验室,湖北武汉430070
2. 华中农业大学园艺植物生物学教育部重点实验室,湖北武汉,430070 |
| |
| 基金项目： | 国家公益性行业(农业)科研专项，中央级公益性科研院所基本科研业务费 |
| |
| 摘 要： | 第二代测序技术,又称"新一代测序技术",其较Sanger测序具有通量高、测序成本低和测序时间短等特点。介绍了以454、Illumina/Solexa和SOLi D为代表的第二代高通量测序技术的基本特性及其在植物全基因组、转录组和miRNA测序等领域的研究进展,并对新一代高通量测序技术的发展趋势进行了展望。
|
| 关 键 词： | 高通量测序 新一代测序 转录组测序 miRNA测序 |
Application of next-generation sequencing technology in plant genetic research |
| |
| LUO Chun,ZHANG Qing-lin,LUO Zheng-rong.Application of next-generation sequencing technology in plant genetic research[J].Guangdong Agricultural Sciences,2015,42(3):186-192. |
| |
| Authors: | LUO Chun ZHANG Qing-lin LUO Zheng-rong |
| |
| Institution: | LUO Chun;ZHANG Qing-lin;LUO Zheng-rong;South Subtropical Crops Research Institute, Chinese Academy of Tropical Agricultural Sciences/Key Laboratory of Tropical Fruit Biology,Ministry of Agriculture;Key Laboratory of Horticultural Plant Biology, Huazhong Agricultural University; |
| |
| Abstract: | The research was carried out to study the effects of four kinds of chemical reagents (KNO3, GA3,KMnO4 and NaOH) on seed germination and seedling growth characteristics of Senna alata L. The results indicated that all concentrations of KMnO4 and GA3 promoted the germination and seeding growth of S. alata L., and 0.1g/LGA3 and 4g/L KMnO4 were the most effective reagents. All KNO3 concentrations promoted the germination, but the growthMof seedlings were not affected. The germination process and shoot height were depressed under different concentrations of NaOH, the larger the concentration was, the more severely the seed germination and seedling |
| |
| Keywords: | |
| 本文献已被 CNKI 万方数据 等数据库收录！ |
| 点击此处可从《广东农业科学》浏览原始摘要信息 |
| 点击此处可从《广东农业科学》下载免费的PDF全文 |
|
