| 白肉火龙果染色体制片技术及核型分析 |
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| 引用本文： | 刘顺枝,刘政浩,林润怡,等.白肉火龙果染色体制片技术及核型分析[J].广东农业科学,2015,42(3):115-118. |
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| 作者姓名： | 刘顺枝 刘政浩 林润怡 等 |
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| 作者单位： | 1. 广州大学生命科学学院,广东广州,510006
2. 广州市果树科学研究所,广东广州,510405 |
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| 基金项目： | 广州市科技计划项目，广州市属高校科研计划项目 |
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| 摘 要： | 以白肉火龙果的实生根为制片材料,在去壁低渗法的基础上改进染色体制片技术,并进行核型分析。结果表明,以白肉火龙果种子在粗砂砾中萌发的实生根为材料,以等体积的0.2%秋水仙素与0.002mol/L8-羟基喹啉预处理1h,在1mol/LHCl中60℃下解离13min,制片效果最好。核型分析显示白肉火龙果的染色体数目为2n=2x=22,全部染色体都为中部染色体,核型公式为2n=2x=22=22m;染色体的相对长度变化范围为6.95%~12.97%,核型为1A型,核不对称系数为52.67%。
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| 关 键 词： | 白肉火龙果 实生根 染色体制片 核型分析 |
Chromosome preparation technique and karyotype analysis of white pitaya (Hylocereus undatus) |
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| LIU Shun-zhi,LIU Zheng-hao,LIN Run-yi,LIN Jian-cong,WANG Yu-lin,XU She-jin,HU Wei-rong.Chromosome preparation technique and karyotype analysis of white pitaya (Hylocereus undatus)[J].Guangdong Agricultural Sciences,2015,42(3):115-118. |
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| Authors: | LIU Shun-zhi LIU Zheng-hao LIN Run-yi LIN Jian-cong WANG Yu-lin XU She-jin HU Wei-rong |
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| Institution: | LIU Shun-zhi;LIU Zheng-hao;LIN Run-yi;LIN Jian-cong;WANG Yu-lin;XU She-jin;HU Wei-rong;School of Life Sciences, Guangzhou University;Guangzhou Fruit Research Institute; |
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| Abstract: | Real root tips of white pitaya (Hylocereus undatus) as material, the chromosome preparation skill was
improved by the wall digestion hypotonic method and the karyotype was analyzed. The results showed that the
optimum chromosome preparation was achieved with root from the germination of seeds on coarse gravels, pretreated
by the mixture of 0.2% colchicine and 0.002 mol/L 8-hydroxyquinoline for 1 h, and dissociated by 1 mol/L HCl for
13 min at 60%. The chromosome number of white pitaya is 2n =2x =22. The 22 chromosomes are all middle
chromosome, so that the karyotype formula of white pitaya is 2n=2x=22=22m, belonging to 1A karyotype. The range
of chromosome relative length is from 6.95%to 12.97%and the karyotype asymmetrical coefficient is 52.67%. |
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| Keywords: | white pitaya real root chromosome preparation karyotype analysis |
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