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Cd胁迫对苦瓜叶片活性氧代谢的影响
引用本文:林新萍,施木田,陈伟,卢婷婷,倪晓超.Cd胁迫对苦瓜叶片活性氧代谢的影响[J].福建农林大学学报(自然科学版),2010,39(2).
作者姓名:林新萍  施木田  陈伟  卢婷婷  倪晓超
作者单位:1. 福建农林大学园艺学院,福建,福州,350002;漳州城市职业学院生物与环境工程系,福建,漳州363000
2. 福建农林大学园艺学院,福建,福州,350002
基金项目:福建省博士后基金资助项目;;福州市科技局资助项目(榕科[2004]121号)
摘    要:以苦瓜为材料,进行砂培研究Cd胁迫对苦瓜叶片活性氧代谢的影响.结果表明:Cd胁迫促使苦瓜叶片O2.-、H2O2产生速率增大,启动膜脂过氧化,从而导致细胞伤害;Cd胁迫促使叶片游离脯氨酸(Pro)积累,起到保护叶片的作用,同时增强抗坏血酸过氧化物酶(APX)、脱氢抗坏血酸还原酶(DR)、谷胱甘肽还原酶(GR)的活性;当Cd2+含量为20 mg.L-1时,DR活性最高,Cd2+含量为80 mg.L-1时,GR活性最高.

关 键 词:镉胁迫  苦瓜  活性氧代谢

Effects of cadmium stress on active oxygen metabolism in leaves of balsam pear
LIN Xin-ping,SHI Mu-tian,CHEN Wei,LU Ting-ting,NI Xiao-chao.Effects of cadmium stress on active oxygen metabolism in leaves of balsam pear[J].Journal of Fujian Agricultural and Forestry University,2010,39(2).
Authors:LIN Xin-ping  SHI Mu-tian  CHEN Wei  LU Ting-ting  NI Xiao-chao
Institution:1.College of Horticulture;Fujian Agriculture and Forestry University;Fuzhou;Fujian 350002;China;2.Biological and Environmental Engineering;Zhangzhou City University;Zhangzhou;Fujian 363000;China
Abstract:Sand culture experiment was used to study the effects of cadmium (Cd) stress on active oxygen metabolism in leaves of balsam pear (Momordica charantia). The results showed that under cadmium stress, the generation of O_2~(-·)and H_2O_2 increased, and initiated lipid peroxidation and damaged the balsam pear leaf cell. Under the stress of cadmium, the content of free proline in balsam pear leaves was cumulated, and it protected balsam pear. Under cadmium stress the activities of ascorbate peroxidase (APX), dehydroascorbate reductase (DR), glutathione reductase (GR) in the leaves of balsom pear enhanced too. But the highest activity of DR was under the concentration of 20 mg·L~(-1) Cd~(2+), and the highest activity of GR was under the concentration of 80 mg·L~(-1) Cd~(2+).
Keywords:cadmium stress  balsam pear  active oxygen metabolism  
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