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| 南方四季杨高效遗传转化体系的建立 | |
| 引用本文： | 王红莉,苏小曼,李甫金,罗建勋,辜云杰,宋鹏.南方四季杨高效遗传转化体系的建立[J].四川林业科技,2012,33(5):46-52. |
| 作者姓名： | 王红莉 苏小曼 李甫金 罗建勋 辜云杰 宋鹏 |
| 作者单位： | 1. 成都市林木种苗站,四川成都,610031 2. 内江市林业局,四川内江,641000 3. 平武县林业局,四川平武,522550 4. 四川省林业科学研究院,四川成都,610081 |
| 基金项目： | 植物资源共享平台建设，省育种攻关-低海拔杨树良种选育致谢:感谢中国林科院国家重点实验室为本实验提供实验条件及技术支持 |
| 摘 要： | 以南方四季杨叶片作为受体材料,分别设置梯度实验,研究了Kan(卡那霉素)选择压以及遗传转化过程中影响转化效率的5个主要因素(预培养时间、浸染时间、共培养温度、共培养时间和乙酰丁香酮浓度),得出30mgL-1的Kan浓度作为南方四季杨遗传转化过程中的选择压。高效遗传转化体系为:叶片预培养3 d;OD600=0.4的菌液浸染10 min～15 min;浸染前菌液中加入AS(乙酰丁香酮)300 umol·L-1;22℃～25℃条件下暗处共培养3d。 |
| 关 键 词： | 南方四季杨 遗传转化 抗性芽 分化率 |
Establishment of the High-performance Genetic Transformation System of Populus detoides ×P.nigra cv.Chile |
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| WANG Hong-li,SU Xiao-man LI Fu-jin LUO Jian-xun GU Yun-jie SONG Peng.Establishment of the High-performance Genetic Transformation System of Populus detoides ×P.nigra cv.Chile[J].Journal of Sichuan Forestry Science and Technology,2012,33(5):46-52. | |
| Authors: | WANG Hong-li SU Xiao-man LI Fu-jin LUO Jian-xun GU Yun-jie SONG Peng |
| Institution: | 1.Chengdu tree seeds and seedings station,Chengdu 610031,Sichuan,China; 2.Neijiang Forestry Bureau,Neijiang 641000,Sichuan,China; 3.Sichuan Academy of Forestry,Chengdu 610081,Sichuan,China;4.Forestry Bureau of Pingwu County,Pingwu 622550) |
| Abstract: | Five main factors affecting transformation of Populus deltoides × P.nigra cv.Chile were studied by Agrobacterium tumefaciens-mediated infecting and experimented with various gradients,such as pre-culture time,infection time,co-culture temperature,co-culture time,acetosyringone（AS） concentration in bacterium fluid;In addition,Kan selection pressure was researched.The result showed that 30 mg·L-1 Kan concentration were suitable.An effective protocol was as follows： 3 days for pre-culture of leaf blades,10 minutes~15 minutes for infection in the OD600=0.4 Agrobacterium tumefaciens bacterium fluid;adding As 300 μmol·L-1 in the bacterium fluid dosage before infection,and 3 days for co-culture in dark under the condition of 22℃~25℃. |
| Keywords: | Populus deltoides×P nigra cv Chile Genetic transformation Resistance buds Differentiation rate |
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