| 磨盘柿与甜柿杂交低结籽率的组织学研究 |
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| 引用本文： | 李壮,扈惠灵,曹永庆,任杰,冷平.磨盘柿与甜柿杂交低结籽率的组织学研究[J].园艺学报,2008,35(5):637-642. |
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| 作者姓名： | 李壮 扈惠灵 曹永庆 任杰 冷平 |
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| 作者单位： | （中国农业大学农学与生物技术学院，北京100094；河南科技学院, 河南新乡453003） |
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| 摘 要： | 通过对西村早生、禅寺丸和花御所3种甜柿自交及与磨盘柿杂交的花粉管荧光观察，磨盘柿胚囊发育的石蜡切片观察，研究了磨盘柿受精率低的原因。结果如下：①磨盘柿与3种甜柿杂交，发育种子比例（发育种子数/胚珠数）明显低于甜柿自交，且父本花粉不同，磨盘柿的结子率有较大差异；②磨盘柿柱头上附着花粉量少，发芽率低，花柱中花粉管数量少，到达基部的条数少。甜柿柱头附着的花粉量大、发芽率高，花柱中花粉管数量大，大部分可以到达子房；③磨盘柿中存在多种异常胚囊，直接或间接的影响受精的顺利进行。较低的受精比率以及大量异常胚囊的存在是导致磨盘柿结籽率低的原因。
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| 关 键 词： | 柿 低结籽率 花粉管 胚囊 石蜡切片 荧光显微技术 |
| 收稿时间： | 2007-12-20 |
| 修稿时间： | 2008-4-1 |
Histological Research on Low Seeded Rate of Hybridization Between 'Mopanshi'and Sweet Persimmon |
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| LI Zhuang,HU Hui-ling,CAO Yong-qing,REN Jie,LENG Ping.Histological Research on Low Seeded Rate of Hybridization Between 'Mopanshi'and Sweet Persimmon[J].Acta Horticulturae Sinica,2008,35(5):637-642. |
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| Authors: | LI Zhuang HU Hui-ling CAO Yong-qing REN Jie LENG Ping |
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| Institution: | (College of Agronomy and Biotechnology, China Agricultural University, Beijing 100094 China; Department of Horticulture, Henan Institute of Science &Technology, Xinxiang, Henan 453003 China) |
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| Abstract: | In order to find out the cause of low fertilization rate of Mopanshi, the pollen germination and pollen tube growth in style on persimmon cultivars Nishimurawase, Zenjimaru, Hanagosho and Mopanshi and the embryo sac development of Mopanshi were observed by means of fluorescence microscopy and with paraffin sections. The results obtained were as follows: ① The ratio of developed seeds (the number of developed seeds: the number of ovules) for Mopanshi crossed with three non-astringent cultivars was distinctly lower than that of self-pollinated three non-astringent cultivars; ② The pollen grains landed on stigma of Mopanshi and their germination rate were fewer than those of three non-astringent cultivars, which indicates that pollen grain germination was affected by female parent and Mopanshi stigma is unsuitable, comparatively for pollen to adhere, hydrate and germinate; ③ Compared with three non-astringent cultivars, there were very fewer Pollen tubes in Mopanshi style which were twisted and curled each other, and furthermore, the portion of pollen tube ceased elongating before they arrived at ovary, while as far as non-astringent cultivars were concerned, abundant pollen tubes could be observed in the ovary; ④ Diverse aberrant embryo sacs were found on Mopanshi, which directly or indirectly affected the normal process of fertilization. In conclusion, poor fertilization and aberrant embryo sac were the main causes of low seeded rate for 'Mopansh' persimmon. |
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| Keywords: | persimmon low fertilization rate pollen tube fluorescence paraffin sections of embryo sac |
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