| 地衣芽孢杆菌生防菌株SDYT-79发酵条件优化 |
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| 引用本文： | 纪明山,王毅婧.地衣芽孢杆菌生防菌株SDYT-79发酵条件优化[J].沈阳农业大学学报,2011,42(2). |
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| 作者姓名： | 纪明山 王毅婧 |
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| 作者单位： | 沈阳农业大学植物保护学院/辽宁省生物农药工程技术研究中心,沈阳,110161 |
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| 基金项目： | 辽宁省高等学校优秀人才支持计划项目(2009R49) |
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| 摘 要： | 探讨地衣芽孢杆菌(Bacillus licheniformis)SDYT-79菌株液体发酵条件,为提高其活性次级代谢产物的产量提供技术支持。通过单因子试验和正交试验,对该菌株的最适发酵培养基成分及发酵条件进行优化。结果表明:地衣芽孢杆菌SDYT-79菌株培养基各组分的最佳配比为:蔗糖3.0%、蛋白胨0.3%、酵母膏2.0%、氯化钠0.3%,最佳发酵培养条件为:初始pH值9.0、培养温度24℃、培养时间36h、250mL三角瓶装液量120mL、接种量体积分数0.5%~1%。在最佳发酵培养基和培养条件下,SDYT-79菌株抑菌能力提高14.3%。
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| 关 键 词： | 地衣芽孢杆菌 发酵条件优化 单因子试验 正交试验 |
Optimizing Fermentation Condition for the Antagonistic Bacillus licheniformis SDYT-79 Strain |
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| JI Ming-shan,WANG Yi-jing.Optimizing Fermentation Condition for the Antagonistic Bacillus licheniformis SDYT-79 Strain[J].Journal of Shenyang Agricultural University,2011,42(2). |
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| Authors: | JI Ming-shan WANG Yi-jing |
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| Institution: | JI Ming-shan,WANG Yi-jing (College of Plant Protection / Engineering Research Center of Biopesticide of Liaoning Province,Shenyang Agricultural University,Shenyang 110161,China) |
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| Abstract: | The research was to investigate the best condition for liquid fermentation of Bacillus licheniformis SDYT-79 strain and increase the antagonistic substance productivity of strain SDYT-79.The single factor experiments and orthogonal experiments were adopted to optimize the liquid fermentation medium and conditions.The constituents of medium,initial pH,fermentation time and temperature were the major factors affecting the yield of SDYT-79.The best compositions of cultural medium were as follow: sucrose 3.0%,p... |
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| Keywords: | Bacillus licheniformis fermentation condition optimization single factor experiment orthogonal experiment |
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