| 氨基隐性孔雀石绿抗体的制备及ELISA方法的建立 |
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| 引用本文： | 常超,张佳艳,程时杰,伍金娥.氨基隐性孔雀石绿抗体的制备及ELISA方法的建立[J].湖北农业科学,2017,56(7). |
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| 作者姓名： | 常超 张佳艳 程时杰 伍金娥 |
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| 作者单位： | 武汉轻工大学食品科学与工程学院,武汉,430023 |
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| 摘 要： | 以N,N-二甲基苯胺、对硝基苯甲醛为原料,合成改造了氨基隐性孔雀石绿半抗原,并通过紫外、红外、质谱鉴定。结果表明,氨基隐性孔雀石绿半抗原改造成功。采用重氮化法合成了氨基隐性孔雀石绿人工抗原,并通过紫外光谱进行鉴定,结果显示偶联蛋白成功。免疫大白兔制备了隐性孔雀石绿抗体,抗体效价达6.4×10~4。建立了隐性孔雀石绿ELISA方法,最低检测线为0.1μg/L。
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| 关 键 词： | 孔雀石绿 隐性孔雀石绿 抗体 ELISA |
Preparation of Antibody of Amino-Leucomalachite Green and Development of ELISA Method |
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| CHANG Chao,ZHANG Jia-yan,CHENG Shi-jie,WU Jin-e.Preparation of Antibody of Amino-Leucomalachite Green and Development of ELISA Method[J].Hubei Agricultural Sciences,2017,56(7). |
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| Authors: | CHANG Chao ZHANG Jia-yan CHENG Shi-jie WU Jin-e |
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| Abstract: | With N,N-dimethylanilin and p-nitrobenzaldehyde as raw materials,the hapten of amino-leucomalachite green (NH2-LMG) was synthesized and then was identified with ultraviolet,infrared and mass spectrum. The results showed that the artificial antigen of NH2-LMG was successfully synthesized by diazotization method and was identified by ultraviolet spectrum, which indicated the success of the coupling protein. The antibody of NH2-LMG was prepared with immune New Zealand white rabbits,and the antibody titer reached 6.4 ×104. The ELISA method was developed, with the limit of detection of 0.1 ug/L. |
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| Keywords: | malachite green leucomalachite green antibody enzyme-linked immunosorbent assay |
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