| 鹅生长激素受体基因荧光定量PCR检测方法的建立 |
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| 作者姓名： | 杨 隽 张 才 肖翠红 李 馨 |
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| 作者单位： | 黑龙江八一农垦大学动物科技学院 黑龙江大庆163319(杨隽，李馨)，吉林大学畜牧兽医学院 吉林长春130062(张才)，黑龙江八一农垦大学生命科技学院 黑龙江大庆163319(肖翠红) |
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| 基金项目： | 黑龙江省教育厅科学技术研究项目;大庆市科技局科技攻关项目 |
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| 摘 要： | 根据GenBank中鹅生长激素受体(GHR)基因序列设计合成了引物和探针,对荧光定量PCR的方法进行了方法学的评估,建立了TaqmanMGB荧光定量RT-PCR检测方法。结果表明,由pMD-18T-GHR所构建的标准曲线线性关系良好,建立的GHR基因荧光定量PCR检测方法灵敏度高、特异性强(可以检测出低于10个拷贝/μl的样品),准确可靠。籽鹅和莱茵鹅GHRmRNA出壳到90日龄生长过程中肝脏中的表达量不同,30日龄不同组织的表达量各有变化特点。
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| 关 键 词： | 鹅 生长激素受体基因 实时荧光定量PCR |
Development of Real-time PCR Assay for Geese Growth Hormone Receptor Gene |
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| Authors: | Yang Jun Zhang Cai Xiao Cuihong Li Xin |
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| Institution: | (1College of Animal Science and Technology, Heilongjiang August First Land Reclamation University, Daqing 163319;
2College of Animal Science and Veterinary Medicine,Jilin University,Changchun 130062;
3College of Life Science and Biotechnology, Heilongjian |
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| Abstract: | The probe and primers were designed and synthesized according to the growth hormone receptor sequence of goose available in GenBank. Then a real-time RT-PCR assay was developed and assessed. Results showed that the standard curve made by pMD-18T-GHR had good linear dependence, and the fluorescent quantitative RT-PCR assay was sensitive, specific and reliable. The expression of GHR mRNA were difference in liver during postnatal 90 days , and there was different changing pattern in other tissues at 30 days between Zigeese and Rhine. |
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| Keywords: | goose growth hormone receptor gene real-time PCR |
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