| 杨树叶片高效离体再生系统的构建 |
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| 作者姓名： | 马同富 马宗新 |
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| 作者单位： | 阜阳师范学院，抗衰老中草药安徽省工程技术研究中心 阜阳236041，安徽省阜阳市农业科学研究所，阜阳236041，阜阳236065 |
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| 摘 要： | 利用速生杨树(Populus.tomentosa Car)叶片为外植体建立离体培养体系,结果表明,叶脉处和叶柄处,极易发生不定芽;芽大多是从叶片上直接产生;培养基中BA浓度是影响不定芽形成的关键;BA浓度在05～2.0mg/L范围内均有不定芽发生,BA1.0mg/L与NAA0.05mg/L搭配有利于不定芽的形成,培养基中添加GA30.1～0.2mg/L可以提高不定芽的发生频率,增殖培养基采用MS BA0.5mg/L NAA0.05mg/L GA30.05mg/L,不仅能够快速增殖,而且芽苗粗壮,玻化率降低。生根过程采用过渡培养效果较好,ABT3生根粉的加入,使试管苗质量大为提高,在此基础上建立起杨树叶片离体再生系统,利用该体系能够获得高的芽苗再生频率,但是不同品种间略有差异。
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| 关 键 词： | 杨树 叶片 培养基 离体再生体系 |
Sdudy on Efficient in Vitro Regeneration System of Leaves of Populus Tomentosa Carr |
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| Authors: | Ma Tongfu Ma Zongxin |
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| Institution: | (1Fuyang Teachers College Fuyang 236041;
2Engineering Technology Research Center of Antiaging Chinese Herb,Anhui Province Fuyang 236041;
3Fuyang Institute of Agricultural Sciences Anhui, Fuyang 236065) |
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| Abstract: | The leaves of fast growing poplar were used as explants and a in vitro culture system was established. The results showed that the leaf vein and petiole produced adventitious buds very easily and most buds occurred directly in the leaves; the BA concentration in media is the key to the bud formation; when the concentration was 0.5~2.0mg/L BA, the adventitious buds occurred, and combining 1.0mg/L BA with0.05mg/l NAA was beneficial to the adventitious bud formation; adding 0.1~0.2mg/L GA3 to the media could increase the Frequency of producing the adventitious buds. Use the propagation medium MS 0.5mg/L BA 0.05mg/L NAA 0.05mg/L GA3 could not only realize rapid propagation but also make the plantlets robust and the verifications rate lower. Adopting transition culture in rooting had a better result, and adding ABT3-rooting-powder to the media made the quality of test-tuber plantlets much better. The in vitro regeneration system of leaves of the poplar was established based on the experimental results. Using this system could reach a high regeneration frequency but different varieties would differ a little bit. |
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| Keywords: | poplar leaf medium in vitro regeneration system |
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