| 鸭胚胎代用蛋壳培养的研究 |
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| 作者姓名： | 张文昌 徐国正 庄益芬 王垚锟 |
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| 作者单位： | 福建农林大学动物科学学院 福州350002 |
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| 基金项目： | 福建省教育厅高校科学技术研究项目 |
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| 摘 要： | 采用两种方法进行了鸭胚胎代用蛋壳培养,旨在提高出雏率。方法Ⅰ将新鲜种蛋的胚胎转移至代用蛋壳Ⅰ内培养,72h后转移至代用蛋壳Ⅱ内培养至出雏。方法Ⅱ将正常孵化72h后的胚胎转移至代用蛋壳Ⅱ后培养至出雏。方法Ⅰ组出雏率为19.2%(10/52);方法Ⅱ组出雏率为40.4%(21/52)。从第6天到出雏同一日龄胚胎存活率方法Ⅰ组显著低于方法Ⅱ组和对照组(P<0.05或P<0.01)。第16天到出雏同一日龄胚胎存活率方法Ⅱ组显著低于对照组(P<0.01)。结果表明方法Ⅰ和方法Ⅱ两种方法均能获得雏鸭,方法Ⅱ优于方法Ⅰ。
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| 关 键 词： | 鸭胚胎 代用蛋壳培养 存活率 |
Culture of Duck Embryo in Surrogate Eggshells |
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| Authors: | Zhang Wenchang Xu Guozheng Zhuang Yifen Wang Yaokun |
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| Institution: | College of Animal Science, Fujian Agriculture and Forestry University, Fuzhou 350002 |
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| Abstract: | To increase the hatchability in vitro,two methods were used to culture the duck embryos in surrogate eggshells.Method one:embryos were transferred into the surrogate eggshell I from the freshly laid eggs,after being cultured for 72hrs,the embryos were transferred into the surrogate eggshell II until hatching.Method two:normal embryos,being incubated for 72 hrs,were transferred into the surrogate eggshell II till to hatching.The results follow:The hatchability of method one and method two was 19.2%(10/52)and 40.4%(21/52),respectively.From 6th day to hatching,the survival rate of embryos with the same cultured time in method one was significantly lower than method two and control(P<0.05 or P<0.01).From 16th day to hatching,the survival rate of embryos with the same cultured time in method two was significantly lower than control(P<0.01).The results indicated that both of the methods can work for the embryo development in vitro,but method two is better. |
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| Keywords: | duck embryo substitutes eggshell culture survival rate |
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