| 披碱草焦磷酸化酶基因EdHP1的功能分析 |
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| 引用本文： | 董建辉,徐晴,周容,盛敏,温亮,龙小玲,樊军,陈明.披碱草焦磷酸化酶基因EdHP1的功能分析[J].湖北农业科学,2017,56(10). |
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| 作者姓名： | 董建辉 徐晴 周容 盛敏 温亮 龙小玲 樊军 陈明 |
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| 作者单位： | 1. 湖北省农业科学院,武汉,430064;2. 中国农业科学院作物科学研究所,北京,100081 |
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| 基金项目： | 国家高技术研究发展计划(863计划)项目，国家自然科学基金项目，湖北省农业科学院青年自然科学基金项目 |
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| 摘 要： | 氢离子焦磷酸化酶(H~+-PPase)是一类H~+转运蛋白,它以焦磷酸(PPi)水解底物,水解PPi产生的自由能与H~+跨膜转运相藕联,将细胞质中的H~+泵入液泡中,建立跨液泡膜电化学梯度,形成质子驱动力,为无机离子及其他溶质进出液泡提供动力,有利于植物维持细胞离子平衡和渗透平衡,增强植物的抗逆性。基于前期对披碱草(Elymus dahuricus)H~+-PPase基因Ed HP1克隆的基础,对其表达谱及基因功能进行了进一步研究。结果表明,通过Northern blot分析发现,该基因在披碱草中受干旱、低温非生物胁迫诱导表达。通过对转Ed HP1基因烟草在干旱、低温环境下的功能分析发现,过表达Ed HP1基因可以提高转基因烟草对干旱、冷胁迫的抗性。
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| 关 键 词： | 披碱草(Elymus dahuricus) 焦磷酸化酶基因 表达分析 干旱 低温 |
Functional Analysis of H+-Pyrophosphatase Gene,EdHP1 of Elymus dahuricus |
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| DONG Jian-hui,XU Qing,ZHOU Rong,SHENG Min,WEN Liang,LONG Xiao-ling,FAN Jun,CHEN Ming.Functional Analysis of H+-Pyrophosphatase Gene,EdHP1 of Elymus dahuricus[J].Hubei Agricultural Sciences,2017,56(10). |
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| Authors: | DONG Jian-hui XU Qing ZHOU Rong SHENG Min WEN Liang LONG Xiao-ling FAN Jun CHEN Ming |
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| Abstract: | H(+)-pyrophosphatase (H+-PPase) is an H+ transporter and taking the pyrophosphate (PPi) as substrate for hy-drolysis. The free energy that produced by hydrolysis of pyrophosphate coupled with the trans-membrane transport of the H+ is able to pump the H+ from the cytoplasm into the vacuole,and form the electro-chemical gradient across the tonoplast and the proton motive force to promote the import and export of the inorganic ions and to maintain the ion balance and osmotic po-tential,finally to enhance the plant tolerance to stress. Based on the H+-PPase gene(EdHP1) had been cloned from E. dahuri-cus in previous studies,a further analysis of this gene,which including the expression and functional analysis of this gene un-der various stress conditions were performed in this study. Northern-blot analysis results showed that the expression of EdHP1 was induced by different abiotic stresses such as drought and cold of E. dahuricus. The functional analyses indicated that over-expression of EdHP1 enhanced the tolerance of transgenic tobacco plants to drought and cold in comparison with wild type plants. |
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| Keywords: | Elymus dahuricus H+-PPase expression analysis drought cold |
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