| 急、慢性冷应激对雏鸡腓肠肌及血清抗氧化功能的影响 |
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| 作者姓名： | 王金涛 李宁 徐世文 |
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| 作者单位： | 东北农业大学动物医学院，黑龙江省农垦科学院畜牧兽医研究所 哈尔滨150038，黑龙江省农垦科学院畜牧兽医研究所，东北农业大学动物医学院，哈尔滨150030，哈尔滨150038，哈尔滨150030 |
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| 摘 要： | 【目的】探讨自由基在冷应激对禽类机体损伤中所起的作用,为探索促进动物冷适应的方法,提高动物的抗寒能力提供一定的理论依据;【方法】应用化学比色法检测了急、慢性冷应激对腓肠肌和血清中总抗氧化能力(T-AOC)、过氧化氢酶(CAT)活性、谷胱甘肽过氧化物酶(GSH-Px)活性、超氧化物歧化酶(SOD)活性及丙二醛(MDA)含量的影响;【结果】急性应激时,随应激时间的延长,腓肠肌T-AOC与SOD活性均逐渐降低,GSH-Px活性、CAT活性及MDA含量均逐渐升高,血清T-AOC逐渐降低,GSH-Px活性、CAT活性、SOD活性及MDA含量均逐渐升高;慢性应激时,随应激时间的延长,腓肠肌T-AOC、GSH-Px活性、CAT活性及SOD活性均逐渐升高,MDA含量逐渐降低,血清T-AOC、GSH-Px活性、CAT活性及MDA含量均逐渐升高,SOD活性逐渐降低;【结论】表明急、慢性冷应激均可使机体的抗氧化功能发生改变,诱发氧化胁迫,导致机体内自由基增多,对机体造成损伤。
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| 关 键 词： | 冷应激 雏鸡 腓肠肌 血清 抗氧化功能 |
Effects of Acute and Chronic Cold Stress on the Antioxidative Function of Gastrocnemius and Serum in Chicken |
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| Authors: | Wang Jintao Li Ning Xu Shiwen |
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| Institution: | 1,College of Veterinary Medicine, Northeast Agricultural University, Harbin 150030; 2,Institute of A nimal Sciences, Heilongjiang Academy of Agricultural Cultivation Sciences, Harbin 150038 |
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| Abstract: | OBJECTIVE] The purpose of the paper is to investigate the role of free radical in the damage of the avian. It can provide a certain theoretical foundation for exploring the method of promoting cold adaptation of the animal and improving the ability of resisting the cold of the animal. METHOD] The chemical colorimetric method is used to detect the change of the T-AOC, CAT activity, GSH-Px activity, SOD activity and MDA contents in gastrocnemius and serum. RESULTS] Both T-AOC and SOD activity gradually decreased, but GSH-Px activity, CAT activity and MDA contents all gradually increased in gastrocnemius in acute cold stress condition. T-AOC gradually decreased, but GSH-Px activity, CAT activity, SOD activity and MDA contents all gradually increased in serum in acute cold stress condition. T-AOC, GSH-Px activity, CAT activity and SOD activity all gradually increased, but MDA contents gradually decreased in gastrocnemius in chronic cold stress condition. T-AOC, GSH-Px activity, CAT activity and MDA contents all gradually increased, but SOD activity gradually decreased in serum in chronic cold stress condition. CONCLUSION] It is demonstrated that acute and chronic cold stress could change antioxidative function, induce oxidative stress, increase the content of free radicals, and finally result in the damage of the body. |
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| Keywords: | Cold stress Chicken Gastrocnemius Serum Antioxidative function |
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