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6种中药对鲫非特异性免疫效果的影响
引用本文:艾春汉,邹金虎,喻运珍,艾桃山.6种中药对鲫非特异性免疫效果的影响[J].淡水渔业,2009,39(1).
作者姓名:艾春汉  邹金虎  喻运珍  艾桃山
作者单位:1. 武汉市水产科学研究所,武汉,430070
2. 武汉中博水产生物技术有限公司,武汉,430070
3. 武汉市水产科学研究所,武汉,430070;武汉中博水产生物技术有限公司,武汉,430070
基金项目:武汉市农科院院管项目 
摘    要:将白芍(Radix Paeoniae Alba),五味子(Fructus Schisandrae),党参(Radix Codonopsis),刺五加(Radix Acan-thopanacis Senticosi),黄芪(Radix Astragali),鱼腥草(Herba Houttuyniae)的醇提取物用生理盐水配成浓度2%的溶液,胸腔注射鲫(Carassius auratus)连续5 d,每次0.2 mL,探讨了6种中药对鲫鱼非特异性免疫效果的影响。结果显示:中药组鲫白细胞吞噬活性明显地高于空白组(P<0.05),几种中药组吞噬活性的高低顺序依次为刺五加、黄芪、党参、五味子、白芍、鱼腥草、空白组。血清溶菌酶和抗菌活力与空白组比较差异显著(P<0.05),其活力高低次序为刺五加、黄芪、鱼腥草、党参。白芍、五味子组溶菌酶活力虽低于空白组,但经统计表明差异不显著(P>0.05)。经嗜水气单胞菌攻毒后各中药组鲫的死亡率均低于空白组。

关 键 词:鲫(Carassius  auratus)  白芍  五味子  党参  刺五加  黄芪  鱼腥草  非特异性免疫

Effect of Six Kinds of Chinese Medicines on Nonspecific Immunity of Carassius auratus
AI Chun-han,ZOU Jin-hu,YU Yun-zhen,AI Tao-shan.Effect of Six Kinds of Chinese Medicines on Nonspecific Immunity of Carassius auratus[J].Freshwater Fisheries,2009,39(1).
Authors:AI Chun-han  ZOU Jin-hu  YU Yun-zhen  AI Tao-shan
Institution:AI Chun-han1,ZOU Jin-hu2,YU Yun-zhen1,2,AI Tao-shan1,2(1.Wuhan Fishery Science Institute,Wuhan 430070,2.Wuhan Chopper Fishery Bio-technology company Limited,Wuhan 430070)
Abstract:In order to study the effect of six kinds of Chinese medicines on nonspecific immunity of Carassius auratus,Alcohol-extract of Radix Paeoniae Alba,Fructus Schisandrae,Radix Codonopsis,Radix Acanthopanacis Senticosi,Radix Astragali,Herba Houttuyniae were prepared to be 2% concentrated solution in isotonic Na chloride.Then injected them to C.auratus′s thoracic cavity 5 days successively,0.2 mL each time.The result showed that the phagocytosis activity of C.auratus white cell in blood in Chinese medicine groups were obviously stronger than that in blank group(P<0.05).The order of phagocytosis activity from high to low among six kinds of Chinese medicine groups was R.Acanthopanacis Senticosi,R.Astragali,R.Codonopsis,F.Schisandrae,R.Paeoniae Alba,H.Houttuyniae and blank group.Compared with the blank group,significant discrepancy was observed on lysozyme activity and antibiosis activity of serum(P<0.05).The order from high to low was R.Acanthopanacis Senticosi,R.Astragali,H.Houttuyniae,R.Codonopsis.Although lysozyme activity in R.Paeoniae Alba and F.Schisandrae groups were lower than that in blank group,there was no significant difference between them through statistics analysis(P<0.05).After conteracting toxic,the mortality of Chinese herbal medicine groups was lower than blank group obviously.
Keywords:Carassius auratus  Radix Paeoniae Alba  Fructus Schisandrae  Radix Codonopsis  Radix Acanthopanacis Senticosi  Radix Astragali  Herba Houttuyniae  nonspecific immunity  
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