| 分蘖数和栽培基质对金钗石斛茎叶生长的影响 |
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| 引用本文： | 郭俊霞,李青苗,吴萍,陈铁柱,胡平,夏燕莉,方清茂.分蘖数和栽培基质对金钗石斛茎叶生长的影响[J].安徽农业科学,2016,44(36). |
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| 作者姓名： | 郭俊霞 李青苗 吴萍 陈铁柱 胡平 夏燕莉 方清茂 |
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| 作者单位： | 四川省中医药科学院,四川成都,610041;四川省中医药科学院,四川成都,610041;四川省中医药科学院,四川成都,610041;四川省中医药科学院,四川成都,610041;四川省中医药科学院,四川成都,610041;四川省中医药科学院,四川成都,610041;四川省中医药科学院,四川成都,610041 |
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| 基金项目： | 四川省基本科研业务专项( A－2014 N－7)。 |
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| 摘 要： | 目的]通过分蘖数、栽培基质对金钗石斛茎叶生长的影响研究,探讨金钗石斛栽培适宜种苗分蘖数和栽培基质,为金钗石斛人工栽培提供科学依据。方法]以贵州赤水一年生金钗石斛组培苗为材料,在人工盆栽条件下,3种分蘖数、4种栽培基质,栽培30、60、90、150、360 d,金钗石斛茎、茎粗生长量、植株叶片数、叶SPAD值、茎鲜重、干重、折干率等形态指标研究。结果]4种栽培基质中,锯末屑是最佳的栽培基质。分蘖数为2,在4种栽培基质中150、360 d茎生长量分别为7.20、5.20、3.20、1.00,8.40、6.80、6.25、2.65 cm,均最高。茎粗的生长量与分蘖数未表现出差异性。3种分蘖数的种苗主茎上叶数的减少量在锯末屑栽培基质中最低,随着栽培时间的增长,叶的减少量越高,叶的SPAD值降低,主茎上叶栽培150、360 d的叶SPAD值,不同分蘖数、不同栽培基质间的差异显著。结论]金钗石斛不同分蘖数种苗栽培360 d,种苗分蘖数与金钗石斛茎、茎粗的生长量、折干率无相关性,与茎的鲜重、干重呈极显著相关。
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| 关 键 词： | 金钗石斛 分蘖数 栽培基质 茎叶 SPAD值 |
Effects of Tiller Number,Cultivation Substrates on the Growth of Stems and Leaves of Dendrobium nobile |
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| Abstract: | Objective] Effects of tiller number, cultivation substrates on the growth of stems and leaves of Dendrobium nobile were studied, the suitable cultivation substrates and tiller number were discussed, so as to provide a scientific basis for artificial cultivation of Dendrobium nobile. Method] Taking Guizhou Chishui annual Dendrobium nobile tissue culture seedlings as materials, under artificial potted conditions, 3 kinds of tiller number, 4 kinds of culture mediums, cultivating for 30, 60, 90,150, 360 d, the growth of stem, stem diameter, leaf number, leaf SPAD value, stem fresh weight, dry weight, branch breaking rate were studied. Result] The sawdust is the best culturing medium. When tiller number is 2, cultivating for 150, 360 d in 4 cultivation matrices, the growth of stem is 7.20, 5.20, 3.20, 1.00, 8.40, 6.80, 6.25, 2.65 cm, were the highest.The growth of stem diameter and tiller number showed no difference.The amount of leaves reduced lowest in the sawdust substrate, leaf SPAD value decreased with time growing and leaf reducing.There were significant differences of leaf SPAD value among different tiller number,various matrices cultivating for 150, 360 d.Conclusion] After seedling cultivation for 360 d of Dendrobium nobile with different tiller number, the growth of stem, stem diameter, branch breaking rate had no correlation with tiller number, while showed significant correlation with stem fresh weight, dry weight. |
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| Keywords: | Dendrobium nobile Tiller number Cultivation substrates Stem leaf SPAD value |
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