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| 嗜肾型鸡传染性支气管炎病毒W93疫苗株S1基因的克隆与序列测定 | |
| 引用本文： | 王文成,赵宝华,尹广东,李尚波,卫广森,许崇波.嗜肾型鸡传染性支气管炎病毒W93疫苗株S1基因的克隆与序列测定[J].中国预防兽医学报,2002,24(5):357-360. |
| 作者姓名： | 王文成 赵宝华 尹广东 李尚波 卫广森 许崇波 |
| 作者单位： | 1. 辽宁省益康生物制品厂,辽宁,辽阳,111000 2. 河北师范大学,生命科学学院,河北,石家庄,050016 3. 宁夏大学,生物工程系,宁夏,银川750021 |
| 摘 要： | 根据国外已发表的鸡传染性支气管炎病毒（IBV）S1基因序列，设计了两对引物并以RT－PCR特异性扩增出嗜肾型IBV W93疫苗株的S1基因，基因产物大小为1.62kb，与设计相符，对其进行序列测定后，与标准毒株H52、H120、M41、BEAU株的S1基因进行同源性比较，结果表明，W93株与H52、H120、M41和BEAU株的核苷酸序列的同源性分别为96．8％、97．1％、96．9％和96．8％，由此可以看出嗜肾型IBV W93疫苗株与标准毒株在S1基因上具有高度的同源性。 |
| 关 键 词： | 嗜肾型 鸡 传染性 支气管炎 病毒 W93疫苗株 S1基因 克隆 序列测定 |
| 文章编号： | 1008-0589(2002)05-0357-04 |
| 修稿时间： | 2002年1月8日 |
Sequencing and Homology Analysis of the S1 Gene of Nephropathogenic Strain W93 |
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| WANG Wen-cheng ,ZHAO Bao-hua ,YIN Guang-dong ,LI Shang-bo ,WEI Guang-sen ,XU Chong-bo.Sequencing and Homology Analysis of the S1 Gene of Nephropathogenic Strain W93[J].Chinese Journal of Preventive Veterinary Medicine,2002,24(5):357-360. | |
| Authors: | WANG Wen-cheng ZHAO Bao-hua YIN Guang-dong LI Shang-bo WEI Guang-sen XU Chong-bo |
| Institution: | WANG Wen-cheng 1,ZHAO Bao-hua 2,YIN Guang-dong 1,LI Shang-bo 1,WEI Guang-sen 2,XU Chong-bo 3 |
| Abstract: | One pair of primers for amplifying the S1 gene of avian infectious bronchitis virus (IBF) had been designed,which the predicted 1.62kb fragment had been amplified via RT-PCR from Nephropathogenic Strain W93.The S1 gene of Nephropathogenic Strain W93 was sequenced and compared with standard strains such as H52,M41,H120 and BEAU.The results showed that the homology of nucleic acids between W93 strain and H52,M41,H120 and BEAU strains was 96.8%,97.1%,96.9% and 96.8% respectively.The high homology of the S1 gene of Nephropathogenic Strain W93 with those of other IBV standard strains had been proved. |
| Keywords: | IBV S1 gene Nucleotide sequence Homology |
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