| 香蕉枯萎病菌生理小种鉴定及其SCAR标记 |
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| 引用本文： | 刘景梅,陈霞,王璧生,何自福,彭埃天,蔡曼珊.香蕉枯萎病菌生理小种鉴定及其SCAR标记[J].植物病理学报,2006,36(1):28-34. |
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| 作者姓名： | 刘景梅 陈霞 王璧生 何自福 彭埃天 蔡曼珊 |
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| 作者单位： | 广东省农业科学院植物保护研究所, 广州 510640 |
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| 基金项目： | 广东省农业科技攻关项目 |
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| 摘 要： | 通过室内人工接种蕉类鉴别寄主,对采集于广东蕉区的18个蕉类枯萎病菌菌株进行鉴定,KP021、KP022、GZ981和JL021 4个菌株属Racel,其余14个菌株属Race4,说明广东蕉区同时存在尖孢镰刀菌古巴专化型Race1和Race4。用RAPD技术对上述18个菌株进行分析,从200条随机引物中筛选出8条引物可产生生理小种RAPD标记12个,其中标记Racel的8个,标记Race4的4个。对这些RAPD标记带分别进行回收、克隆、测序,根据这些特异片段序列分别设计相应的SCAR引物,通过对18个菌株的PCR扩增检验,有4个RAPD标记成功地转化为SCAR标记,其中Race1-SCAR标记1个、Race4-SCAR标记2个、同时能鉴定出2个小种的SCAR标记1个。应用这4个SCAR标记同时对采自田间的9个病菌分离物进行检测,能够准确地鉴定出广东蕉区的尖孢镰刀菌古巴专化型Racel和Race4,这为下一步开展香蕉枯萎病菌生理小种的分子鉴定及各生理小种田间流行动态监测奠定了基础。
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| 关 键 词： | 香蕉枯萎病 尖孢镰刀菌古巴专化型 RAPD标记 SCAR标记 |
| 文章编号： | 0412-0914(2006)01-0028-07 |
| 修稿时间： | 2005年1月5日 |
Race identification and SCAR markers development for the pathogen of banana Panama disease Fusarium oxysporum f. sp. cubense |
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| LIU Jing-mei,CHEN Xia,WANG Bi-sheng,HE Zi-fu,PENG Ai-tian,CAI Man-shan.Race identification and SCAR markers development for the pathogen of banana Panama disease Fusarium oxysporum f. sp. cubense[J].Acta Phytopathologica Sinica,2006,36(1):28-34. |
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| Authors: | LIU Jing-mei CHEN Xia WANG Bi-sheng HE Zi-fu PENG Ai-tian CAI Man-shan |
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| Institution: | Institute of Plant Protection, Guangdong Academy of Agricultural Sciences, Guangzhou 510640, China |
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| Abstract: | The pathogenicity of 18 strains of Fusarium oxysporum f. sp. cubense (FOC) from banana-growing regions in Guangdong province were identified on the differential hosts. The strain KP021 ,KP022,GZ981 and JL021 belonged to race 1 ,and the other strains belonged to race 4. A total of 200 oligonucleotides were used as primers to perform random amplified polymorphic DNA (RAPD) analysis, eight of them could amplify racespecific DAN fragments. Based on the cloning and sequencing of these RAPD marker fragments, the sequence characterized amplified region (SCAR) primers were designed respectively. The PCR amplification of the 18 FOC isolates and 9 new field isolates with SCAR primers showed that 4 SCAR markers could be specifically used to separate race 1 and/or race 4. These results provide a possible approach to identify the physiological races of FOC by molecular techniques and to monitor the epidemic trend of the 2 races in the fields. |
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| Keywords: | banana Panama disease Fusarium oxysporum f sp cubense RAPD marker SCAR marker |
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