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| 利用SSR分析小豆种质遗传多样性 | |
| 引用本文： | 宫慧慧,谢华,马荣才,曹鸣庆,万平,金文林,濮邵京.利用SSR分析小豆种质遗传多样性[J].农业生物技术学报,2008,16(5). |
| 作者姓名： | 宫慧慧 谢华 马荣才 曹鸣庆 万平 金文林 濮邵京 |
| 作者单位： | 1. 首都师范大学生命科学学院,北京,100037;北京市农林科学院农业生物技术研究中心,北京,100097 2. 北京市农林科学院农业生物技术研究中心,北京,100097 3. 北京农学院农学系,北京,100081 |
| 摘 要： | 摘要：小豆是一类重要的食用豆，本研究利用45对SSR引物对小豆基因组DNA进行了SSR标记的筛选鉴定，共筛选出多态性良好、扩增效果稳定的SSR引物18对。利用筛选出的18个SSR标记，分析了来自我国栽培小豆优异种质53份和日本引进种质27份，旨在阐明其遗传多样性特点，为育种利用提供理论依据。结果表明，在所有参试的80份小豆种质中共鉴定出等位变异92个，平均每个位点为5.1个；其中53份国内小豆和27份日本小豆的等位变异数分别为89个和74个，平均每个位点分别为4.9个和4.1个。所有供试小豆平均每个位点的多态信息含量（PIC）为0.64，变化范围为0.23～0.83，其中国内小豆的平均PIC值为0.63，变化范围为0.23~0.86；日本小豆平均PIC值为0.61，变化范围为0.20~0.81。国内栽培小豆和日本小豆在等位变异数、多态信息含量（PIC）、遗传相似性系数均存在差异，UPGMA聚类分析将参试的80份小豆明显分为五大类，聚类结果与小豆种质地理起源呈现出一定的相关性。试验表明，在小豆遗传育种中，可以通过种质资源相互利用来拓宽育成品种的遗传基础，同时这些SSR标记对于小豆资源DNA指纹图谱构建、遗传作图、基因型鉴定及分子标记辅助育种具有重要意义。 |
| 关 键 词： | SSR 遗传多样性 小豆[Vigna angularis (Willd.) Ohwi & Ohashi] |
| 收稿时间： | 2008-2-25 |
| 修稿时间： | 2008-4-12 |
Genetic Diversity Assessment of Azuki Bean by SSR Markers |
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| GONG Hui-hui,XIE Hua,MA Rong-cai,CAO Ming-qing,WAN Ping,JIN Wen-lin,PU Shao-jing.Genetic Diversity Assessment of Azuki Bean by SSR Markers[J].Journal of Agricultural Biotechnology,2008,16(5). | |
| Authors: | GONG Hui-hui XIE Hua MA Rong-cai CAO Ming-qing WAN Ping JIN Wen-lin PU Shao-jing |
| Abstract: | ABSTRACT: Azuki bean Vigna angularis (Willd.) Ohwi & Ohashi] is an important legume crop grown for its protein rich grains. Firstly, 45 microsatellite DNA markers were used to amplify genomic DNAs from 80 accessions of azuki bean, including 53 accessions of Chinese azuki bean and 27 accessions of Japanese azuki bean in this work. As a result, 18 pairs of SSR primers could amplify polymorphic single-locus SSR from all of these materials, and these polymorphic markers were then used to analyze genetic diversity of azuki bean. A total of 92 alleles was detected, among them 89 and 74 alleles were detected in 57 accessions of Chinese azuki bean and 27 accessions of Japanese azuki bean respectively. The average number of alleles per locus was 5.4 for all the accessions, 4.9 for Chinese azuki bean, and 4.1 for Japanese azuki bean. The polymorphic information content (PIC) of each SSR locus varied from 0.23 to 0.83 with an average of 0.64 in all the accessions, from 0.23 to 0.86 with an average of 0.63 in the Chinese azuki bean, and from 0.20 to 0.81 with an average of 0.61 in the Japanese azuki bean. Genetic differences between the Chinese and Japanese azuki bean were detected for specific alleles, PIC and pairwise genetic similarity. UPGMA cluster analysis of the similarity data basically separates the eighty accessions into five groups. A dendrogram based on the microsatellite analysis generally agreed with the pedigree of the azuki bean accessions. These results indicated that Chinese and Japanese azuki bean germplasm could be used for broadening genetic base of current cultivars, and the SSR markers should be useful for genetic mapping, genotype identification, and marker-assisted selection of azuki bean. |
| Keywords: | SSR |
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