| 基于全基因组SNP高效鉴定燕麦种质资源 |
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| 引用本文： | 徐金青,边海燕,王寒冬,王 蕾,张 波,尤 恩,李晓兰,陈文杰,沈裕虎.基于全基因组SNP高效鉴定燕麦种质资源[J].麦类作物学报,2022(6):677-684. |
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| 作者姓名： | 徐金青 边海燕 王寒冬 王 蕾 张 波 尤 恩 李晓兰 陈文杰 沈裕虎 |
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| 作者单位： | (1.中国科学院高原适应与进化重点实验室/青海省作物分子育种重点实验室/青藏高原作物种质资源研究与利用实验室,中国科学院西北高原生物研究所，青海西宁 810001; 2.中国科学院种子创新研究院,青海西宁 810001; 3.中国科学院大学生命科学学院,北京 100049） |
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| 基金项目： | 第二次青藏高原综合科学考察研究项目（2019QZKK0303）；青海省自然科学基金团队项目(2022-ZJ-902);青海省创新平台建设专项(2022-ZJ-Y04)；中国科学院“西部之光”交叉团队项目-重点实验室合作研究专项 |
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| 摘 要： | 为探究鉴别燕麦品种特异性所需SNP标记的最少数量与鉴别效果，以25个生产上大面积推广的栽培燕麦品种为材料，利用燕麦Illumina Inc.iSelect 6K微珠芯片进行基因分型，按照具有多态性、最小等位基因频率（MAF）大于0.05、缺失率小于0.50的条件进行筛选，获得2 214个高质量的SNP标记。所有SNP标记的平均MAF为0.75，平均多态性信息含量（PIC）为0.28。群体遗传结构分析将25个燕麦品种划分为5个类群。通过去冗余分析，获得8个能够高效鉴别燕麦参试品种的SNP标记。这8个SNP标记的平均MAF为0.62，平均PIC为0.35，表现出丰富的遗传多样性。测序结果显示，所筛选的8个SNP标记具有较好的稳定性和重现性。进一步利用这8个SNP标记构建了25个燕麦栽培品种的SNP指纹图谱，为燕麦栽培品种真实性鉴定和纯度检测提供了可用的标记组合。
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| 关 键 词： | 燕麦(Avena sativa L.) SNP 品种鉴定 DNA指纹图谱 |
Effective Identification for Oat Varieties by Genome-Wide SNPs |
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| XU Jinqing,BIAN Haiyan,WANG Handong,WANG Lei,ZHANG Bo,YOU En,LI Xiaolan,CHEN Wenjie,SHEN Yuhu.Effective Identification for Oat Varieties by Genome-Wide SNPs[J].Journal of Triticeae Crops,2022(6):677-684. |
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| Authors: | XU Jinqing BIAN Haiyan WANG Handong WANG Lei ZHANG Bo YOU En LI Xiaolan CHEN Wenjie SHEN Yuhu |
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| Abstract: | In order to explore the minimum number and identification effect of SNP (single nucleotide polymorphism) markers needed to distinguish the oat varieties (Avena sativa L.) specificity,25 oat cultivars that have been widely planted were used as materials.Oat Illumina Inc.iSelect 6K beadchip array was used for genotyping.According to the conditions of polymorphism,minimum allele frequency (MAF) greater than 0.05 and deletion rate less than 0.50,2 214 high-quality SNP markers were obtained.The average MAF of all SNP markers was 0.75 and the average polymorphism information content (PIC) was 0.28.The 25 oat cultivars were divided into five groups by population genetic structure analysis.Eight SNP markers were obtained by the redundancy analysis,which could effectively identify the tested oat varieties.The average MAF of eight SNP markers was 0.62 and the average PIC was 0.35,showing rich genetic diversity.The sequencing results showed that the eight SNP markers screened had good stability and reproducibility.In this study,SNP fingerprints of the 25 oat cultivars were provided,which provided a useful marker combination for authenticity identification and purity detection of oat cultivars. |
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| Keywords: | Oats(Avena sativa L ) SNP Variety identification DNA fingerprint |
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