| 三株拮抗细菌的鉴定及抑菌作用研究 |
| |
| 引用本文： | 王树雪,魏艳敏,尚巧霞,赵晓燕,刘正坪.三株拮抗细菌的鉴定及抑菌作用研究[J].中国生物防治,2013(4):647-654. |
| |
| 作者姓名： | 王树雪 魏艳敏 尚巧霞 赵晓燕 刘正坪 |
| |
| 作者单位： | 北京农学院植物科学技术学院／农业应用新技术北京市重点试验室,北京102206 |
| |
| 基金项目： | 基金项目：北京市属高等学校人才强教计划资助项目（PHR201107135）;科研基地建设-科技创新平台-害虫综合防治创新平台建设（PXM2013-014207_000044）;国家自然科学基金（31201559） |
| |
| 摘 要： | 为了充分发掘和利用有益微生物资源，本文以草莓根腐尖孢镰刀菌Fusariumoxysporum为靶标菌，从草莓根际土壤中分离筛选得到3株拮抗细菌，通过培养性状与形态特征、生理生化特征以及16SrDNA同源序列比对分析进行了鉴定，菌株1-9和9—4为多粘类芽孢杆菌Paenibacilluspolymyxa、菌株12—4为枯草芽孢杆菌Bacillussubtilis。这3株细菌对草莓根腐尖孢镰刀菌的抑菌作用进行了初步的研究，结果表明，3株拮抗细菌对草莓根腐尖孢镰刀菌的生长有很好的抑制作用，镜下观察对菌丝有致畸作用，其培养滤液还能够抑制草莓根腐病尖孢镰刀菌分生孢子的产生与萌发。
|
| 关 键 词： | 拮抗细菌 草莓根腐病 尖孢镰刀菌 细菌鉴定 抑菌作用 |
Identification and Antifungal Activity of Three Antagonistic Bacteria Strains |
| |
| WANG Shuxue,WEI Yanmin,SHANG Qiaoxia,ZHAO Xiaoyan,LIU Zhengping.Identification and Antifungal Activity of Three Antagonistic Bacteria Strains[J].Chinese Journal of Biological Control,2013(4):647-654. |
| |
| Authors: | WANG Shuxue WEI Yanmin SHANG Qiaoxia ZHAO Xiaoyan LIU Zhengping |
| |
| Institution: | (Beijing Key Laboratory of New Technology in Agricultural Application/College of Plant Science and Technology, Beijing University of Agriculture, Beijing 102206, China) |
| |
| Abstract: | Three antifungal bacteria were isolated from strawberry rhizosphere soil with strawberry root rot pathogen Fusarium oxysporum as target plant pathogen. The three strains were identified according to their culture characteristic, morphology, physiological and biochemical property and 16s rDNA sequence analysis. The results showed that strains 1-9 and 9-4 were identified as Paenibacillus polymyxa, and 12-4 was identified as Bacillus subtilis. The three strains of antagonistic bacteria exhibited inhibition activity to the hypha growth, morphology and sporulation against F. oxysporum. The culture filtrate of the three antifungal strains also inhibited the conidial germination ofF. oxysporum. |
| |
| Keywords: | antagonistic bacterium strawberry root rot Fusarium oxysporum bacterium identification antifungalactivity |
| 本文献已被 维普 等数据库收录！ |
