| 黑曲霉固态发酵产酸性磷酸酯酶的工艺研究 |
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| 引用本文： | 刘继强,柯雪琴,邬敏辰.黑曲霉固态发酵产酸性磷酸酯酶的工艺研究[J].安徽农业科学,2007,35(7):1905-1907. |
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| 作者姓名： | 刘继强 柯雪琴 邬敏辰 |
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| 作者单位： | 江南大学生物工程学院,江苏无锡,214036;江南大学医学系,江苏无锡,214064 |
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| 摘 要： | 为探讨黑曲霉固态发酵酸性磷酸酯酶(ACP)的产酶条件,首次采用固态培养的方法,在对黑曲霉3350产酸性磷酸酯酶的培养基组成和培养条件单因素研究的基础上,设计了Plackett-Burman试验选取了3个主要影响因素,并进行响应面分析.结果表明,黑曲霉3350产酸性磷酸酯酶的最适培养基和发酵条件为:麸皮8.0 g,蔗糖2.0 g,豆饼粉1.11 g,水13.9 ml,土温-801.0ml/kg,NH4NO320g/kg,KH2PO42.5g/kg,自然pH值;32℃固体静置培养120h,期间翻曲2～3次.在该条件下菌株最高产酶活性为154IU/g干曲,比优化前产酶提高88%,与液态发酵周期(9d)相比缩短了44%.
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| 关 键 词： | 黑曲霉 酸性磷酸酯酶 固态发酵 响应面分析 |
| 文章编号： | 0517-6611（2007）07-01905-03 |
| 修稿时间： | 2006-12-02 |
Technics Research of Solid Fermentation for Acid Phosphatase Production by Aspergillus Niger |
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| LIU Ji-qiang et al.Technics Research of Solid Fermentation for Acid Phosphatase Production by Aspergillus Niger[J].Journal of Anhui Agricultural Sciences,2007,35(7):1905-1907. |
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| Authors: | LIU Ji-qiang |
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| Institution: | School of Bioengineering, Southern Yangtze University, Wuxi, Jiangsu 214036 |
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| Abstract: | The research on the nutrition ingredients and fermentation conditions on the production of acid phosphatase by Aspergillus niger 3350 showed that the optimal fermentation medium and condition contained 8.0 g wheat bran,2.0 g sucrose, 1.11g soybean flour,13.9 ml H_2O,soil temperature-80 of 1.0 ml/kg,20 g/kg, KH_2PO_4 2.5 g/kg,CaCl_2 1.0 g/kg,MgSO_4 1.0 g/kg,nature pH,solid culture for 20 h at 32 ℃,turning over the aspergillus 2 times.Under these conditions,the highest acid phosphatase activity was 154 IU/g dry matter,being 88% higher than acid phosphatase production before optimization. |
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| Keywords: | Aspergillus niger Acid phosphatase Solid-state fermentation Response surface analysis |
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