| 野猪源奇异变形杆菌的分离鉴定及致病性分析 |
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| 引用本文： | 刘妍罕,曾茂芹,张飘,杨霞,杨颖,程振涛,粟海军,文明.野猪源奇异变形杆菌的分离鉴定及致病性分析[J].中国动物传染病学报,2022(1):44-52. |
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| 作者姓名： | 刘妍罕 曾茂芹 张飘 杨霞 杨颖 程振涛 粟海军 文明 |
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| 作者单位： | 贵州大学动物科学学院;贵州省动物生物制品工程技术研究中心;贵州大学林学院 |
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| 基金项目： | 国家林业与草原局非洲野猪防控野猪专项调查贵州专项(2019);贵州省科技平台及人才团队计划项目(黔科合平台人才[2018]5253号)。 |
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| 摘 要： | 为探究贵州省野猪携带病原菌情况,本试验对无菌采集自全省6个地区15头野猪肝脏样本的分离菌株进行培养观察、革兰染色、生化鉴定、16S rRNA基因序列分析、药敏试验和小鼠致病性试验及病理组织切片,同时根据已报道的基因序列合成奇异变形杆菌的mrpA、rsbA、ureC、zapA、rsmA、hpmA、FIiL、ucaA、pm...
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| 关 键 词： | 野猪 奇异变形杆菌 分离鉴定 毒力基因 |
Isolation,Identifi cation and Pathogenicity of Proteus mirabilis Isolates from Wild Boars |
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| LIU Yanhan,ZENG Maoqin,ZHANG Piao,YANG Xia,YANG Ying,CHENG Zhentao,SU Haijun,WEN ming.Isolation,Identifi cation and Pathogenicity of Proteus mirabilis Isolates from Wild Boars[J].Chinese Journal of Animal Infectious Diseases,2022(1):44-52. |
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| Authors: | LIU Yanhan ZENG Maoqin ZHANG Piao YANG Xia YANG Ying CHENG Zhentao SU Haijun WEN ming |
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| Institution: | (College of Animal Science,Guizhou University,Guiyang 550025,China;Research Center of Engineering Technology for Animal Biological Products of Guizhou,Guiyang 550025,China;College of Forestry,Guizhou University,Guiyang 550025,China) |
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| Abstract: | This experiment was carried out for isolation and identifi cation of Proteus mirabilis from 15 wild boar liver samples from 6 regions of Guizhou province.Total 6 P.mirabilis isolates were obtained through culture observation,gram staining,biochemical reaction,16S rRNA gene sequence analysis and drug susceptibility.The colony morphology,biochemical characteristics and 16S rRNA gene sequence of these isolated were consistent with P.mirabilis.The dominant colony staining showed a single array of Gram-negative cocci.Biochemical testing of these isolates revealed decomposition of glucose to produce acid and gas,production of hydrogen sulfi de,positive reaction of urea and lysine,and negative reaction for peptone water.The 16S rRNA fragments of these 6 isolates were 1453 bp in length and had more than 98%homology with P.mirabilis in the published accounts.In addition,10 virulence gene primers of P.mirabilis(mrpA,rsbA,ureC,zapA,rsmA,hpmA,FIiL,ucaA,pmfA,atfA)were designed according to the reported sequences and used in PCR amplifi cation.All these virulence genes existed in these 6 isolates,suggesting they were highly pathogenic.Drug susceptibility testing showed that these 6 isolates were resistant to a variety of antibiotics but sensitive to norfl oxacin,ofl oxacin,amikacin,fl orfenicol.Additional experiment results also showed that these isolates were pathogenic to mice.These results from the present study provided a basis for the epidemiological investigation of P.mirabilis infection in wild boars in Guizhou. |
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| Keywords: | Wild boar Proteus mirabilis isolation and identifi cation virulence gene epidemiological investigation |
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