| 猪源化脓隐秘杆菌分离的鉴定与病原特性研究 |
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| 引用本文： | 姜轩,徐承倩,张欣,张奥,赵瑞利,马吉飞,孙英峰.猪源化脓隐秘杆菌分离的鉴定与病原特性研究[J].中国动物传染病学报,2022(1):53-58. |
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| 作者姓名： | 姜轩 徐承倩 张欣 张奥 赵瑞利 马吉飞 孙英峰 |
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| 作者单位： | 天津农学院动物科学与动物医学学院 |
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| 基金项目： | 天津市生猪产业技术体系创新团队-猪细菌病岗位项目(ITTPRS2017004)。 |
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| 摘 要： | 从天津地区某猪场病猪肺脏脓肿中分离到一株具有β溶血特性的细菌,经形态学与生化特性分析,初步确定为化脓隐秘杆菌(Arcanobacterium pyogenes),并命名为TJjh1913.利用PCR扩增16S rRNA基因,经Blast序列分析,其序列与GenBank数据库中猪源化脓隐秘杆菌的核苷酸序列同源性高达99....
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| 关 键 词： | 猪 化脓隐秘杆菌 分离鉴定 病原特性 |
Isolation and Identifi cation of Arcanobacterium pyogenes of Swine Origin and Pathogenicity in Mice |
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| JIANG Xuan,XU Chengqian,ZHANG Xin,ZHANG Ao,ZHAO Ruili,MA Jifei,SUN Yingfeng.Isolation and Identifi cation of Arcanobacterium pyogenes of Swine Origin and Pathogenicity in Mice[J].Chinese Journal of Animal Infectious Diseases,2022(1):53-58. |
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| Authors: | JIANG Xuan XU Chengqian ZHANG Xin ZHANG Ao ZHAO Ruili MA Jifei SUN Yingfeng |
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| Institution: | (College of Animal Science and Animal Medicine,Tianjin Agricultural University,Tianjin 300384,China) |
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| Abstract: | One bacterial strain TJjh1913 withβhemolytic activity was isolated from lung abscess of a pig in Tianjin area and identifi ed as Arcanobacterium pyogenes by morphological and biochemical analyses.Then the 16S rRNA genes were amplifi ed by PCR and sequence was analyzed by Blast software.The result showed that the 16S rRNA sequence of TJjh1913 strain was 99.9%homologous with that of Arcanobacterium pyogenes in published GenBank.Further analysis revealed that it carried 3 virulence genes Plo,NanH and CbpA.In addition,the bacterial culture was inoculated into Kunming mice for testing its pathogenicity and LD50 dose was determined to be 105 CFU/mL.Drug susceptibility testing indicated that this strain was highly sensitive to cephalosporins and fluoroquinolones and resistant to aminoglycoside.These results provided useful information on identifi cation,epidemiological investigation and control of porcine A.pyogenes. |
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| Keywords: | Swine Arcanobacterium pyogenes isolation and identifi cation pathogenicity |
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