| A型塞内卡病毒VP1蛋白的原核表达及其多克隆抗体的制备 |
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| 引用本文： | 农作荣,王豪,牛晨霞,全东群,曾悦,任同伟,王玉旭,陈樱,欧阳康,黄伟坚,韦祖樟.A型塞内卡病毒VP1蛋白的原核表达及其多克隆抗体的制备[J].中国动物传染病学报,2022(1):120-126. |
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| 作者姓名： | 农作荣 王豪 牛晨霞 全东群 曾悦 任同伟 王玉旭 陈樱 欧阳康 黄伟坚 韦祖樟 |
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| 作者单位： | 广西大学动物科学技术学院动物传染病与分子免疫学实验室 |
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| 基金项目： | 国家自然科学基金(31972666)。 |
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| 摘 要： | A型塞内卡病毒(SVA)是一种新发传染病病原.研究旨在对SVA的衣壳蛋白VP1进行原核表达,并制备其多克隆抗体.以SVA广西分离株SVA-GX01(GenBank登录号:MK039162)RNA为模版,通过RT-PCR扩增结构蛋白VP1基因,构建重组质粒pET-32a-VP1并转化表达菌株大肠杆菌BL21(DE3)进行...
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| 关 键 词： | A型塞内卡 结构蛋白 VP1 原核表达 多克隆抗体 |
Prokaryotic Expression of the VP1 Protein of Senecavirus A and Preparation of Its Polyclonal Antibodies |
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| NONG Zuorong,WANG Hao,NIU Chenxia,QUAN Dongqun,ZENG Yue,REN Tongwei,WANG Yuxu,CHEN Ying,OUYang Kang,HUANG Weijian,WEI Zuzhang.Prokaryotic Expression of the VP1 Protein of Senecavirus A and Preparation of Its Polyclonal Antibodies[J].Chinese Journal of Animal Infectious Diseases,2022(1):120-126. |
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| Authors: | NONG Zuorong WANG Hao NIU Chenxia QUAN Dongqun ZENG Yue REN Tongwei WANG Yuxu CHEN Ying OUYang Kang HUANG Weijian WEI Zuzhang |
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| Institution: | (Laboratory of Animal infectious Diseases and molecular Immunology,College of Animal Science and Technology,Guangxi University,Nanning 530005,China) |
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| Abstract: | Senecavirus A is a new infectious agent.The aim of this study was to express the VP1 protein of Senecavirus A(SVA)and prepare its polyclonal antibodies.The structural protein of SVA VP1 gene was amplifi ed by RT-PCR using the SVA RNA of SVA strain as the template.The amplicon was cloned into the prokaryotic expression vector pET-32a,resulting a recombinant plasmid pET-32a-SVA-VP1.The plasmid was transformed into E.coli BL21(DE3)competent cells which were then induced.The purifi ed VP1 protein was used to inject the New Zealand White rabbits to prepare its polyclonal antibodies.The polyclonal antibodies against VP1 in BHK-21 cells infected with SVA was analyzed by Western blot and indirect immunofl uorescence assay.The results showed that the recombinant SVA VP1 protein was expressed as inclusion bodies and soluble solid in E.coli BL21(DE3)cells.This polyclonal antibodies effectiveness to be determined up to 1:64000.Results of western blot and indirect immunofl uorescence assay showed that the polyclonal antibodies could interact specifi cally with VP1 of SVA.Its polyclonal antibodies provided valuable biomaterials for the establishment of serological detection method for SVA. |
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| Keywords: | Senecavirus A(SVA) capsid protein VP1 prokaryotic expression polyclonal antibody |
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