Intraspecific diversity of <Emphasis Type="Italic">Undaria pinnatifida</Emphasis> (Harvey) Suringar (Laminariales,Phaeophyta) from Japan,China and Korea,based on the <Emphasis Type="Italic">cox1</Emphasis> gene and ITS2 sequences |
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| Authors: | Hirotoshi Endo Eun-Jeong Park Youichi Sato Hiroyuki Mizuta Naotsune Saga |
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| Institution: | (1) Faculty of Fisheries Sciences, Hokkaido University, Hakodate Hokkaido, 041-8611, Japan;(2) Riken Food Co., Ltd, Tagajyo Miyagi, 985-8540, Japan;(3) Present address: Seaweed Research Center, National Fisheries Research and Development Institute, Mokpo, 530-831, Republic of Korea |
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| Abstract: | As a trial to develop a method of authenticating the place of origin of circulated Undaria pinnatifida products, we investigated their intraspecific genetic diversity using the mitochondrial cytochrome c oxidase subunit 1 gene
(cox1) and the internal transcribed spacer 2 (ITS2) region of the nuclear ribosomal DNA (rDNA) sequence. Four dried U. pinnatifida products labeled with their origins (one from Japan, one from China and two from Korea), natural plants collected from three
locations (two from Japan and one from China), and cultivated plants collected from two locations (one from Japan and one
from China) were used in the present study. The amplified fragments of cox1 were 664 bp in length, and the aligned sequences were highly homologous. Among the nine sequences, no insertions or deletions
were found and six substitution positions were detected, and they were classified into five haplotypes. In contrast, multiple
highly variable regions were found in ITS2, and some of them carried a restriction site for Mbo II. Polymerase chain reaction-restriction fragment length polymorphism analysis showed different restricted profiles among
the tested samples. The availability of molecular markers for authenticating food products of U. pinnatifida is discussed. |
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| Keywords: | Intraspecific diversity PCR-RFLP Undaria pinnatifida |
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