| 半夏多糖提取工艺优化及其清除自由基能力研究 |
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| 引用本文： | 郭辉娟,魏定国.半夏多糖提取工艺优化及其清除自由基能力研究[J].安徽农业科学,2010,38(34):19341-19342,19345. |
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| 作者姓名： | 郭辉娟 魏定国 |
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| 作者单位： | 河北大学生命科学学院,河北保定,071002;河北大学生命科学学院,河北保定,071002 |
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| 摘 要： | 目的]研究半夏Pinellia ternate（Thunb.）Breit.]多糖的提取工艺及其清除自由基的能力。方法]在单因素试验的基础上,通过正交试验确定纤维素酶法提取半夏多糖的最佳条件;采用羟自由基体系对半夏多糖清除羟自由基的能力进行研究。结果]纤维素酶法提取半夏多糖的最优条件为：提取温度55℃,提取时间3.5 h,pH值为4.5,加酶量为4%。多糖浓度为10 mg/ml时,其对羟自由基的清除率达到71.75%。结论]得到了纤维素酶法提取半夏多糖的最佳条件,探明了半夏多糖对羟自由基的清除作用。
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| 关 键 词： | 半夏 多糖 提取 纤维素酶 羟自由基 |
Study on Optimization of Extraction Process of Pinellia ternate (Thunb.) Breit. Polysaccharide and Its Scavenging Effect on Radical |
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| Institution: | GUO Hui-juan et al(College of Life Sciences,Hebei University,Baoding,Hebei 071002) |
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| Abstract: | Objective]To study the extraction process of Pinellia ternate(Thunb.) Breit.polysaccharide.Method]Based on single factor test,the extraction conditions were optimized by orthogonal test.The scavenging capacity of Pinellia ternate(Thunb.) Breit.polysaccharide was investigated using hydroxyl free radical system.Result]he optimal extraction conditions were as follows: extraction temperature of 55 ℃,extraction duration of 3.5 h,pH value of 4.5 and cellulose amount of 4%.As concentration of polysaccharide was 10 mg/ml,its scavenging rate on radical reached 71.55%.Conclusion]The optimum extraction technology of Pinellia ternate(Thunb.) Breit.polysaccharide with cellulase extraction was obtained in this study,and it had strong scavenging effect on hydroxyl radical. |
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| Keywords: | Pinellia ternate(Thunb ) Breit Polysaccharide Extraction Cellulase Free radical |
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