| Comparative Analyses of Proteome Complement Between Worker Bee Larvae of High Royal Jelly Producing Bees （A. m. ligustica） and Carniolian Bees （A. m. camica） |
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| 作者姓名： | CHEN Jian LI Jian-ke |
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| 作者单位： | Institute of Apicultural Research, Chinese Academy of Agricultural Sciences~Key Laboratory of Pollinating Insect Biology, Ministry ofAgriculture, Beijing 100093, P.R, China |
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| 基金项目： | This paper is translated from its Chinese version in Scientia Agricultura Sinica.Acknowledgements This work is supported by a special fund of Technical Production System of the National Beekeeping Industry, China （NYCYTX-43）, the National Natural Science Foundation of China （30972148）, and Special Scientific and Research Found for Public Welfare Industry,China （nyhyzx07-041）. |
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| 摘 要： | This study is to compare the protein composition of the high royal jelly producing bee （A. m. ligustica） with that of Carniolian bee （A. m. carnica） during their worker larval developmental stage. The experiment was carried out by two- dimensional gel electrophoresis. The results showed that significant higher numbers of total proteins （283） were detected in larvae of high royal jelly producing bees （Jelly bee） than those of Carniolian bees （152） on 2-d-old larvae. Among them, 110 proteins were presented on both strains of bee larvae, whereas 173 proteins were specific to larvae of Jelly bees, and 42 proteins were exclusive to Carniolian larvae. However, on the 4th d, a significant higher number of total proteins （290） were detected in larvae of Jelly bees than those of Carniolian bees （240）, 163 proteins resolved to both bee larvae, and 127 proteins were specific to Jelly bees and 77 proteins to Camiolian bees. Until the 6th d, also a significant higher number of total proteins （236） were detected in larvae of Jelly bees than those of Carniolian bees （180）, 132 proteins were constantly expressed in two bee larvae, whereas 104 and 48 proteins are unique to Jelly bee and Carniolian bee larvae, respectively. We tentatively concluded that the metabolic rate and gene expression of Jelly bees larvae is higher than those of Carniolian bees based proteins detected as total proteins and proteins specific to each stage of two strains of bee larvae. Proteins constantly expressed on 3 stages of larval development with some significant differences between two bee strains, and proteins unique to each stage expressed differences in term of quality and quantity, indicating that larval development needed house keeping and specific proteins to regulate its growth at different development phage, but the expression mold is different between two strains of larval development.
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| 关 键 词： | 蜜蜂 饲养 品种 管理 |
Comparative Analyses of Proteome Complement Between Worker Bee Larvae of High Royal Jelly Producing Bees （A. m. ligustica） and Carniolian Bees （A. m. camica） |
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| CHEN Jian,LI,Jian-ke.Comparative Analyses of Proteome Complement Between Worker Bee Larvae of High Royal Jelly Producing Bees （A. m. ligustica） and Carniolian Bees （A. m. camica）[J].Agricultural Sciences in China,2009,8(10):1219-1227. |
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| Institution: | 1. Department of Bioresource Sciences, Andong National University, Andong GB 760–749, Republic of Korea;2. Research Institute of Luminous Organisms, 2749 Nakanogo (Hachijojima), Tokyo 100–1623, Japan;3. Department of Genetics and Physiology, Oulu University, Oulu FIN 90140, Finland |
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| Abstract: | This study is to compare the protein composition of the high royal jelly producing bee (A. m. ligustica) with that of Carniolian bee (A. m. carnica) during their worker larval developmental stage. The experiment was carried out by two-dimensional gel electrophoresis. The results showed that significant higher numbers of total proteins (283) were detected in larvae of high royal jelly producing bees (Jelly bee) than those of Carniolian bees (152) on 2-d-old larvae. Among them, 110 proteins were presented on both strains of bee larvae, whereas 173 proteins were specific to larvae of Jelly bees, and 42 proteins were exclusive to Carniolian larvae. However, on the 4th d, a significant higher number of total proteins (290) were detected in larvae of Jelly bees than those of Carniolian bees (240), 163 proteins resolved to both bee larvae, and 127 proteins were specific to Jelly bees and 77 proteins to Carniolian bees. Until the 6th d, also a significant higher number of total proteins (236) were detected in larvae of Jelly bees than those of Carniolian bees (180), 132 proteins were constantly expressed in two bee larvae, whereas 104 and 48 proteins are unique to Jelly bee and Carniolian bee larvae, respectively. We tentatively concluded that the metabolic rate and gene expression of Jelly bees larvae is higher than those of Carniolian bees based proteins detected as total proteins and proteins specific to each stage of two strains of bee larvae. Proteins constantly expressed on 3 stages of larval development with some significant differences between two bee strains, and proteins unique to each stage expressed differences in term of quality and quantity, indicating that larval development needed house keeping and specific proteins to regulate its growth at different development phage, but the expression mold is different between two strains of larval development. |
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| Keywords: | honeybee （Apis mellifera） worker bee larva two-dimensional gel electrophoresis proteome |
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