| 蛋白核酸合成抑制剂对甜菜谷氨酰胺合成酶活性调控分析 |
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| 引用本文： | 陈胜勇,李彩凤,侯静,马凤鸣,孙世臣.蛋白核酸合成抑制剂对甜菜谷氨酰胺合成酶活性调控分析[J].东北农业大学学报,2009,40(1). |
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| 作者姓名： | 陈胜勇 李彩凤 侯静 马凤鸣 孙世臣 |
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| 作者单位： | 东北农业大学农学院,哈尔滨,150030 |
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| 基金项目： | 国家自然科学基金，黑龙江省博士后科研启动基金 |
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| 摘 要： | 在氮素诱导下,进行甜菜谷氨酰胺合成酶(GS)活性的测定,筛选出最佳的氮素诱导处理,研究核酸合成抑制剂放线菌素D和蛋白合成抑制剂放线菌酮对GS在酶活水平上的表达调控。结果表明,NO3-:NH4+=80:20处理时,GS活性最高,并且在此处理下放线菌素D和放线菌酮均能抑制GS活性。说明甜菜GS的表达可能在转录水平和翻译水平上均受到调控。
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| 关 键 词： | 甜菜 谷氨酰胺合成酶 放线菌素D(ActD) 放线菌酮(CHX) |
Regulation of inhibitors of protein synthesis and nucleic acid synthesis on glutamine synthetase activity in sugar beet (Beta vulgaris L.) |
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| CHEN Shengyong,LI Caifeng,HOU Jing,MA Fengming,SUN Shichen.Regulation of inhibitors of protein synthesis and nucleic acid synthesis on glutamine synthetase activity in sugar beet (Beta vulgaris L.)[J].Journal of Northeast Agricultural University,2009,40(1). |
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| Authors: | CHEN Shengyong LI Caifeng HOU Jing MA Fengming SUN Shichen |
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| Institution: | College of Agriculture;Northeast Agricultural University;Harbin 150030;China |
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| Abstract: | The regulation of actinomycin D(inhibitor of nucleic acid synthesis) and cycloheximide (inhibitor of protein synthesis) on glutamine synthetase(GS) activity in sugar beet was studied under the nitrogen inducement. The best nitrogen treatment was chosen, and GS activity was determined. The results showed that the GS activity was the highest under treatment of NO3-: NH4+=80: 20 and GS activity was inhibited by actinomycin D and cycloheximide under this treatment. This indicates that the expression of GS in su... |
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| Keywords: | sugar beet(Beta vulgaris L ) glutamine synthetase(GS) actinomycin D(Act D) cycloheximide (CHX) |
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