| 马鹿茸不同部位IGF-1含量的比较分析 |
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| 引用本文： | 郝林琳,刘松财,任晓慧,戴建威,王伟,张永亮.马鹿茸不同部位IGF-1含量的比较分析[J].经济动物学报,2005,9(4):201-203. |
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| 作者姓名： | 郝林琳 刘松财 任晓慧 戴建威 王伟 张永亮 |
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| 作者单位： | 吉林大学农学部畜牧兽医学院,长春,130062;吉林大学农学部畜牧兽医学院,长春,130062;河北农业大学水产学院,秦皇岛,066003 |
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| 基金项目： | 本溪天山鹿业新技术有限公司合作项目 |
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| 摘 要： | 采用醋酸溶液和乙醇提取了6支马鹿茸的主杠尖部、中部、根部3个部位的多肽,进行了IGF-1的SDS-PAGE和Western blot分析,采用RIA法测定了马鹿茸酸粗提物及醇提多肽的IGF-1含量。结果表明,酸粗提物中IGF-1存在游离和与结合蛋白结合两种形式,醇提物中只存在分子量为7.5 ku的游离IGF-1。马鹿茸不同部位酸粗提物和醇提物之间IGF-1含量经RIA法检测表明,主杠尖部、中部、根部3个部位IGF-1含量差异显著,其中尖部IGF-1含量分别比中部和根部高43.10%(P<0.01)、284.83%(P<0.01),中部比根部高168.93%(P<0.01);经醇提后,各部位之间IGF-1含量差异显著,其中尖部IGF-1含量分别比中部和根部高42.33%(P<0.05)、164.04%(P<0.01),中部比根部高85.51%(P<0.05)。该试验为马鹿茸的分段利用提供了内在质量上的依据。
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| 关 键 词： | 马鹿茸 多肽 胰岛素样生长因子1 比较分析 |
| 文章编号： | 1007-7448(2005)04-0201-03 |
| 收稿时间： | 2005-06-27 |
| 修稿时间： | 2005年6月27日 |
Comparative Analysis of IGF- 1 Contents in Different Segments of Cervus elaphus linnaeus |
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| HAO Lin-lin,LIU Song-cai,REN Xiao-hui,DAI Jian-wei,WANG Wei,ZHANG Yong-liang.Comparative Analysis of IGF- 1 Contents in Different Segments of Cervus elaphus linnaeus[J].Journal of Economic Animal,2005,9(4):201-203. |
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| Authors: | HAO Lin-lin LIU Song-cai REN Xiao-hui DAI Jian-wei WANG Wei ZHANG Yong-liang |
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| Institution: | 1. College of Animal Science and Veterinary Medicine, Jilin University, Changchun 130062, China; 2. College of Aquaculture, Hebei Agricultural University, Qinhuangdao 066003, China |
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| Abstract: | In this study,acid extract and alcohol extract of six cervus elaphus linnaeus were obtained.Insulin-like growth factor 1(IGF-1)was identified by SDS-PAGE and Western blot.IGF-1 contents in different segments(top、middle & basis,three segments)were detected with RIA.The results of SDS-PAGE and Western blot showed that IGF-1 was extracted with acid and alcohol.IGF-1 contents of the acid extract in top was higher 43.10%(P<0.01)and 284.83%(P<0.01)than in middle and basis respectively,was higher 42.33%(P<0.05)and 164.04%(P<0.01) for alcohol extract polypeptide.IGF-1 contents of the acid extract in middle was higher 168.93%(P<0.01) than in basis,was higher 85.51%(P<0.05) for alcohol extract polypeptide.This test offered an internal quality index in subsection utilization of cervus elaphus linnaeus. |
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| Keywords: | Cervus elaphus linnaeus polypeptide IGF - 1 comparative analysis |
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