| 韩国栗疫病抗病品种的遗传变异分析 |
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| 引用本文： | 朴春根,Kim Kyung-hee,Lee Sang-hyun,Lee Seung-kyu.韩国栗疫病抗病品种的遗传变异分析[J].植物保护学报,2007,34(2):153-156. |
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| 作者姓名： | 朴春根 Kim Kyung-hee Lee Sang-hyun Lee Seung-kyu |
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| 作者单位： | 中国林业科学研究院森林生态环境与保护研究所国家林业局森林保护学重点实验室，Division of Forest Insect Pests and Diseases Korea Forest Research Institute，Seoul130-712，Korea，Division of Forest Insect Pests and Diseases，Korea Forest Research Institute，Seoul130-712，Korea，Division of Forest Insect Pests and Diseases，Korea Forest Research Institute，Seoul130-712，Korea，北京100091 |
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| 基金项目： | 韩国高等教育财团国际合作交流项目 |
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| 摘 要： | 为了分析韩国栗疫病的抗病品种和感病品种的遗传变异和抗病性的筛选,利用抗病性的快速检测法和RAPD(random amplified polymorphic DNA)方法对13个栗树品种进行了抗病性检测和RAPD标记分析。抗病性的快速检测选出了5个抗病品种、5个感病品种和3个中度抗病(或中度感病)品种,并且这一结果与该品种的田间表现相一致。利用筛选的12个随机引物,扩增了100个多态性RAPD片段,但未发现与抗病性或感病性相关的特异RAPD片段。聚类分析结果表明,12个品种大致分为抗病、感病和中度抗病(或中度感病)等3个大组,并与抗病性的快速检测结果基本一致。抗病品种“MANSEKI”表现出了相对于12个品种较远的亲缘关系。
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| 关 键 词： | 栗树 栗疫病菌 遗传变异 |
| 收稿时间： | 2006-08-03 |
| 修稿时间： | 2006-08-03 |
Genetic variation of chestnut cultivars for resistance to Cryphonectria parasitica in Korea |
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| PIAO Chun-gen,Kim Kyung-hee,Lee Sang-hyun and Lee Seung-kyu.Genetic variation of chestnut cultivars for resistance to Cryphonectria parasitica in Korea[J].Acta Phytophylacica Sinica,2007,34(2):153-156. |
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| Authors: | PIAO Chun-gen Kim Kyung-hee Lee Sang-hyun and Lee Seung-kyu |
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| Institution: | 1. Research Institute of Forest Ecology, Environment and Protection, Chinese Academy of Forestry, The Key Open Laboratory of State Forestry Administration, The Laboratory of Forest Protection, Beijing 100091, China; 2. Department of Forest Diseases and Pests, Korea Forest Research Institute, Seoul 130-712, Korea |
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| Abstract: | Resistance of 13 chestnut cultivars(Castanea spp.) to Cryphonectria parasitica in Korea was determined by the rapid testing method. Five chestnut cultivars are resistant, five cultivars susceptible and three cultivars moderate resistant (or susceptible).The genetic variation was investigated by the random amplified polymorphic DNA(RAPD), 12 random primers were selected in RAPD analysis, 100 polymorphic RAPD bands were amplified, but the peculiar RAPD band which discriminates between resistant and susceptible cultivars could not be detected. For the linkage result of cluster analysis, 12 cultivars could be classified as the resistant, susceptible and moderate resistant (or susceptible) groups approximately, which are almost coordinated to the above rapid testing results. The genetic relationship between one resistant cultivar and other 12 cultivars is very low. These results suggest that.the primers selection of genetic regions related to resistance will be effective for the research of genetic variation and identification of resistance in chestnut. |
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| Keywords: | Castanea spp Cryphonectria parasitica genetic variation |
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