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德系黑白花奶牛数量性状基因定位研究初报
引用本文:徐宁迎,傅衍.德系黑白花奶牛数量性状基因定位研究初报[J].浙江大学学报(农业与生命科学版),1999(1).
作者姓名:徐宁迎  傅衍
作者单位:浙江大学动物科学学院
摘    要:在德系黑白花奶牛中,对产奶量、乳蛋白量、乳脂量和奶中体细胞数四个数量性状进行基因定位,试验采用祖孙女设计法。试验动物为16个父系半同胞家系共755头种公牛,试验共测定了8对染色体上的64颗微卫星,父代公牛四个性状的育种值由孙代母牛估算得到。连锁分析采用回归间距定位法,临界F值由置换试验法得到。

关 键 词:数量性状  间距定位法  微卫星  祖孙女设计法  置换试验法

Preliminary study of mapping quantitative trait loci on the German Holstein dairy cattle. Journal of Zhejiang Agricultural University
Xu Ningying ,Fu Yan ,Reinsch N ,Looft C ,Kalm E.Preliminary study of mapping quantitative trait loci on the German Holstein dairy cattle. Journal of Zhejiang Agricultural University[J].Journal of Zhejiang University(Agriculture & Life Sciences),1999(1).
Authors:Xu Ningying  Fu Yan  Reinsch N  Looft C  Kalm E
Institution:Xu Ningying 1,Fu Yan 1,Reinsch N 2,Looft C 2,Kalm E 2
Abstract:A total of 755 bulls belonging to 16 half sib families of German Holstein dairy cattle were genotyped for 64 microsatellite markers on 8 chromosomes. The data were used in an attempt to map quantitative trait loci applying regression as interval mapping approach. For association analysis with a granddaughter design, the estimated breeding values for 4 traits were used: milk yield, protein yield, fat yield and somatic cell count. The empirical values of significance thresholds were determined using a permutation test on the experimental data.
Keywords:QTL  interval mapping  microsatellite  granddaughter design  permutation test  
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