| 烟草蚀纹病毒山东分离物全基因组序列的克隆和保守性分析 |
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| 引用本文： | 张林,韩全军,袁彤彤,宋云枝,朱常香,温孚江.烟草蚀纹病毒山东分离物全基因组序列的克隆和保守性分析[J].植物保护学报,2011,38(5):401-407. |
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| 作者姓名： | 张林 韩全军 袁彤彤 宋云枝 朱常香 温孚江 |
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| 作者单位： | 山东农业大学,作物生物学国家重点实验室,山东省作物生物学重点实验室,泰安271018 |
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| 基金项目： | 国家自然科学基金(30771408) |
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| 摘 要： | 为利用RNA介导的病毒抗性策略,培育抗性稳定或抗多烟草蚀纹病毒（Tobacco etch virus,TEV）株系的转基因植株,采用RT-PCR及5＇-RACE方法克隆了烟草蚀纹病毒山东分离物TEV-SD1的全基因组序列。TEV-SD1全基因组核苷酸序列长度为9494 bp,包含1个9165 bp的开放阅读框架（open reading frame,ORF）,编码3054个氨基酸。将TEV-SD1基因组序列与GenBank中已公布的4个TEV全基因组序列和11个外壳蛋白（coat protein,CP）基因序列比对分析发现,各分离物CP基因间的核苷酸和氨基酸序列平均相似性分别为96.65%和98.31%,高于其它功能基因间的相似性;各分离物CP基因3＇端核苷酸序列相似性平均为96.55%,高于5＇端序列。聚类分析发现TEV在自然界中的分子变异与其寄主关系密切。
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| 关 键 词： | 烟草蚀纹病毒 基因克隆 外壳蛋白 序列分析 RNA干扰 |
| 收稿时间： | 2011/3/24 0:00:00 |
Sequence cloning and conservative analysis of the genomic RNAs of Tobacco etch virus SD1 isolate |
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| Zhang Lin,Han Quanjun,Yuan Tongtong,Song Yunzhi,Zhu Changxiang and Wen Fujiang.Sequence cloning and conservative analysis of the genomic RNAs of Tobacco etch virus SD1 isolate[J].Acta Phytophylacica Sinica,2011,38(5):401-407. |
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| Authors: | Zhang Lin Han Quanjun Yuan Tongtong Song Yunzhi Zhu Changxiang and Wen Fujiang |
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| Institution: | State Key Laboratory of Crop Biology, Shandong Key Laboratory of Crop Biology, Shandong Agricultural University, Tai'an 271018, Shandong Province, China;State Key Laboratory of Crop Biology, Shandong Key Laboratory of Crop Biology, Shandong Agricultural University, Tai'an 271018, Shandong Province, China;State Key Laboratory of Crop Biology, Shandong Key Laboratory of Crop Biology, Shandong Agricultural University, Tai'an 271018, Shandong Province, China;State Key Laboratory of Crop Biology, Shandong Key Laboratory of Crop Biology, Shandong Agricultural University, Tai'an 271018, Shandong Province, China;State Key Laboratory of Crop Biology, Shandong Key Laboratory of Crop Biology, Shandong Agricultural University, Tai'an 271018, Shandong Province, China;State Key Laboratory of Crop Biology, Shandong Key Laboratory of Crop Biology, Shandong Agricultural University, Tai'an 271018, Shandong Province, China |
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| Abstract: | |
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| Keywords: | Tobacco etch virus genomic cloning coat protein sequence analysis RNA interference |
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