| 二化螟盘绒茧蜂寄生对寄主二化螟幼虫免疫反应的影响 |
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| 引用本文： | 李秀花,姚洪渭,叶恭银.二化螟盘绒茧蜂寄生对寄主二化螟幼虫免疫反应的影响[J].植物保护学报,2011,38(4):313-319. |
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| 作者姓名： | 李秀花 姚洪渭 叶恭银 |
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| 作者单位： | 浙江大学昆虫科学研究所, 农业部作物病虫分子生物学重点开放实验室, 杭州 310029;浙江大学昆虫科学研究所, 农业部作物病虫分子生物学重点开放实验室, 杭州 310029;浙江大学昆虫科学研究所, 农业部作物病虫分子生物学重点开放实验室, 杭州 310029 |
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| 基金项目： | 公益性行业(农业)科研专项(200803004) |
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| 摘 要： | 为了探明二化螟盘绒茧蜂Cotesia chilonis (Matsumura)寄生对寄主二化螟Chilo suppressalis (Walker)幼虫血细胞和体液免疫反应的影响,观察并测定了二化螟盘绒茧蜂寄生引起二化螟幼虫血细胞数量、延展、存活、吞噬和包囊作用以及血淋巴酚氧化酶活性等的变化。结果显示,二化螟幼虫血细胞总数在寄生后1天即显著高于对照。其中,颗粒血细胞和浆血细胞在寄生后的数量变化均与血细胞总数变化相似,颗粒血细胞在寄生后3天、浆血细胞在寄生后0.5天起即分别与其对照差异显著,但两种血细胞在血细胞总数中各自所占的比例与对照多无显著差异。二化螟幼虫血细胞延展能力在寄生后0.5天受到显著抑制,此后与对照无显著差异;同时寄生可增加寄主血细胞的死亡率。寄主幼虫血细胞的吞噬作用在寄生后2天起显著降低;包囊作用则在寄生后1天起显著降低。二化螟幼虫被二化螟盘绒茧蜂寄生后,血淋巴酚氧化酶活性显著升高,但随后呈逐渐下降趋势。研究结果说明,二化螟盘绒茧蜂寄生使寄主二化螟幼虫的免疫反应呈现一定的规律变化。
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| 关 键 词： | 二化螟盘绒茧蜂 二化螟 寄生 免疫反应 |
| 收稿时间： | 2011/3/14 0:00:00 |
Effects of parasitization by Cotesia chilonis (Hymenoptera: Braconidae) on larval immune responses of Chilo suppressalis (Lepidoptera: Pyralidae) |
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| Li Xiuhu,Yao Hongwei and Ye Gongyin.Effects of parasitization by Cotesia chilonis (Hymenoptera: Braconidae) on larval immune responses of Chilo suppressalis (Lepidoptera: Pyralidae)[J].Acta Phytophylacica Sinica,2011,38(4):313-319. |
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| Authors: | Li Xiuhu Yao Hongwei and Ye Gongyin |
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| Institution: | Ministry of Agriculture Key Laboratory of Molecular Biology of Crop Pathogens and Insects, Institute of Insect Sciences, Zhejiang University, Hangzhou 310029, Zhejiang Province, China;Ministry of Agriculture Key Laboratory of Molecular Biology of Crop Pathogens and Insects, Institute of Insect Sciences, Zhejiang University, Hangzhou 310029, Zhejiang Province, China;Ministry of Agriculture Key Laboratory of Molecular Biology of Crop Pathogens and Insects, Institute of Insect Sciences, Zhejiang University, Hangzhou 310029, Zhejiang Province, China |
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| Abstract: | To explore the effects of parasitization by Cotesia chilonis (Matsumura) on larval immune reactions of Chilo suppressalis (Walker), the changes in the total number, spreading, viability, phagocytosis and encapsulation capacity of hemocytes, and phenoloxidase activity of hemolymph in parasitized larvae of C.suppressalis were observed and measured. The total number of hemocytes in parasitized larvae became significantly higher than that of unparasitized control from 1 day post-parasitization. The numbers of granulocytes and plasmatocytes in parasitized larvae, showed a similar pattern of variation with the total number of hemocytes, began significantly higher at 3 days and 0.5 day post-parasitization, respectively. However, the percentages of these two types of hemocytes in the total number of hemocytes from parasitized larvae were almost not significantly different with their unparasitized controls. The spreading behavior of host hemocytes was markedly inhibited at 0.5 day post-parasitization. After then, it showed no significant difference with the unparasitized control. The mortality of hemocytes in host larvae increased after oviposition. Hemocytes phagocytosis of parasitized larvae reduced significantly at 2 days post-parasitization, while the encapsulation capacity of hemocytes reduced significantly at 1 day post-parasitization. It showed a dramatic increase with further decrease in phenoloxidase activity of hemolymph in parasitized larvae. The results suggest that the parasitization by C.chilonis may result in some regular changes of immunoreactions of its host C.suppressalis. |
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| Keywords: | Cotesia chilonis Chilo suppressalis parasitism immune response |
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