| 噻唑蓝法和刃天青法检测植物成分对两种病原细菌的抑菌活性 |
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| 引用本文： | 袁高庆,黎起秦,秦健,叶云峰,林纬.噻唑蓝法和刃天青法检测植物成分对两种病原细菌的抑菌活性[J].植物保护学报,2011,38(4):306-312. |
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| 作者姓名： | 袁高庆 黎起秦 秦健 叶云峰 林纬 |
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| 作者单位： | 广西大学农学院, 南宁 530004;广西大学农学院, 南宁 530004;广西大学农学院, 南宁 530004;广西大学农学院, 南宁 530004;广西药用植物园, 南宁 530023;广西大学农学院, 南宁 530004 |
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| 基金项目： | 国家自然科学基金(31060236) |
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| 摘 要： | 通过比较茄青枯拉尔氏菌和柑橘黄单胞菌柑橘致病变种菌量、噻唑蓝和刃天青的浓度、抑菌剂与菌体作用时间等因素对吸光值的影响,优化噻唑蓝和刃天青法检测抑菌剂抑菌活性的条件,即:细菌初始浓度为105~106 CFU/mL,噻唑蓝和刃天青浓度分别为0.5 mg/mL和0.02 mg/mL,抑菌剂作用时间16~24 h。运用优化的噻唑蓝和刃天青法检测了植物提取物和分离物对2种细菌的抑菌活性,2种方法得到的结果一致。以茄青枯拉尔氏菌为靶标菌,对于无色化合物EE-1和土黄色化合物EE-2,2种方法测定的最低抑菌浓度相同,分别为10 μg/mL和20 μg/mL。表明噻唑蓝和刃天青法能快速、准确地测定微量植物成分的抑菌活性。
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| 关 键 词： | 噻唑蓝 刃天青 植物成分 抑菌活性 |
| 收稿时间： | 9/2/2010 12:00:00 AM |
Methythiazol tetrazolium and resazurin assays for detection of antibacterial activities of plant constituents against two bacterial pathogens |
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| Yuan Gaoqing,Li Qiqin,Qin Jian,Ye Yunfeng and Lin Wei.Methythiazol tetrazolium and resazurin assays for detection of antibacterial activities of plant constituents against two bacterial pathogens[J].Acta Phytophylacica Sinica,2011,38(4):306-312. |
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| Authors: | Yuan Gaoqing Li Qiqin Qin Jian Ye Yunfeng and Lin Wei |
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| Institution: | College of Agriculture, Guangxi University, Nanning 530004, Guangxi Zhuang Autonomous Region, China;College of Agriculture, Guangxi University, Nanning 530004, Guangxi Zhuang Autonomous Region, China;College of Agriculture, Guangxi University, Nanning 530004, Guangxi Zhuang Autonomous Region, China;College of Agriculture, Guangxi University, Nanning 530004, Guangxi Zhuang Autonomous Region, China;Guangxi Botanical Garden of Medicinal Plants, Nanning 530023, Guangxi Zhuang Autonomous Region, China;College of Agriculture, Guangxi University, Nanning 530004, Guangxi Zhuang Autonomous Region, China |
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| Abstract: | Optimized conditions of methythiazol tetrazolium and resazurin assays for detection of antibacterial activities of bactericides were established by evaluating influences of the concentration of Ralstonia solanacearum and Xanthomonas citri pv.citri,the concentration of methythiazol tetrazolium and resazurin,and reaction time of bactericide with bacteria on the optical density.The results showed that optimized conditions of methythiazol tetrazolium and resazurin assays were that bacterial concentration was 10... |
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| Keywords: | methythiazol tetrazolium resazurin plant constituent antibacterial activity |
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