| 水稻品种抗黑条矮缩病人工接种鉴定方法 |
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| 引用本文： | 周彤,王英,吴丽娟,范永坚,周益军.水稻品种抗黑条矮缩病人工接种鉴定方法[J].植物保护学报,2011,38(4):301-305. |
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| 作者姓名： | 周彤 王英 吴丽娟 范永坚 周益军 |
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| 作者单位： | 南京农业大学植物保护学院, 南京 210014;江苏省农业科学院植物保护研究所, 江苏省植物病毒病诊断检测技术服务中心, 南京 210095;南京农业大学植物保护学院, 南京 210014;江苏省农业科学院植物保护研究所, 江苏省植物病毒病诊断检测技术服务中心, 南京 210095;江苏省农业科学院植物保护研究所, 江苏省植物病毒病诊断检测技术服务中心, 南京 210095;江苏省农业科学院植物保护研究所, 江苏省植物病毒病诊断检测技术服务中心, 南京 210095;江苏省农业科学院植物保护研究所, 江苏省植物病毒病诊断检测技术服务中心, 南京 210095 |
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| 基金项目： | 江苏省自然科学基金(BK2009325),国家公益性行业(农业)科研专项(201003031, nyhxzx07-51),国家自然科学基金 (31101412),江苏省支撑计划项目(BE2009385),江苏省农业科技自主创新资金项目(cx(10)414) |
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| 摘 要： | 为建立科学有效的水稻品种抗黑条矮缩病人工接种鉴定方法,分别研究了病毒在介体体内的循回时间、接种时间、接种强度、水稻接种苗龄4个因素对鉴定效果的影响。结果显示,病毒在介体体内的循回时间为12~15天或21~24天条件下,鉴定效果优于8~11天和16~17天处理;接种48~72 h条件下,鉴定效果优于12~24 h处理;有效接种强度4~20虫/苗条件下,鉴定效果优于1~3虫/苗处理;水稻接种苗龄0.5~2.5叶龄条件下,鉴定效果优于2.5~3.5叶龄处理。由此构建了水稻抗黑条矮缩病人工接种鉴定方法:循回时间为12~15天、接种时间48~72 h、有效接种强度4~20虫/苗和水稻接种苗龄0.5~2.5叶。在此条件下对不同抗性表现的水稻品种进行鉴定,其鉴定结果与重病区田间鉴定没有显著性差异,表明所建立的人工接种鉴定方法能客观地反映水稻品种对黑条矮缩病的抗性水平。
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| 关 键 词： | 水稻黑条矮缩病 水稻品种 人工接种 抗性鉴定 |
| 收稿时间： | 2011/3/11 0:00:00 |
Method of artificial inoculation identification of rice cultivar resistance to rice black-streaked dwarf |
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| Zhou Tong,Wang Ying,Wu Lijuan,Fan Yongjian and Zhou Yijun.Method of artificial inoculation identification of rice cultivar resistance to rice black-streaked dwarf[J].Acta Phytophylacica Sinica,2011,38(4):301-305. |
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| Authors: | Zhou Tong Wang Ying Wu Lijuan Fan Yongjian and Zhou Yijun |
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| Institution: | College of Plant Protection, Nanjing Agricultural University, Nanjing 210014, Jiangsu Province, China;Technical Service Center for Diagnosis and Detection of Plant Viruses, Institute of Plant Protection, Jiangsu Academy of Agricultural Sciences, Nanjing 210095, Jiangsu Province, China;College of Plant Protection, Nanjing Agricultural University, Nanjing 210014, Jiangsu Province, China;Technical Service Center for Diagnosis and Detection of Plant Viruses, Institute of Plant Protection, Jiangsu Academy of Agricultural Sciences, Nanjing 210095, Jiangsu Province, China;Technical Service Center for Diagnosis and Detection of Plant Viruses, Institute of Plant Protection, Jiangsu Academy of Agricultural Sciences, Nanjing 210095, Jiangsu Province, China;Technical Service Center for Diagnosis and Detection of Plant Viruses, Institute of Plant Protection, Jiangsu Academy of Agricultural Sciences, Nanjing 210095, Jiangsu Province, China;Technical Service Center for Diagnosis and Detection of Plant Viruses, Institute of Plant Protection, Jiangsu Academy of Agricultural Sciences, Nanjing 210095, Jiangsu Province, China |
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| Abstract: | In order to develop an effective method for artificial inoculation identification of resistance to Rice black-streaked dwarf virus(RBSDV) in rice varieties,the effects of four factors,including virus latent period in vectors,inoculation time,inoculation intensity and stage of the seedlings for inoculating,were evaluated in this paper.The results revealed that the effects of artificial inoculation identification for 12-15 days' or 21-24 days' virus latent period in vectors treatment were better than that for... |
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| Keywords: | rice black-streaked dwarf rice varieties artificial inoculation method resistance identification |
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