A comparative assessment of the utility of PCR-based marker systems in pearl millet |
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| Authors: | Mahendar Thudi Senapathi Senthilvel Andrew Bottley C Tom Hash Arjula R Reddy Alex F Feltus Andrew H Paterson David A Hoisington Rajeev K Varshney |
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| Institution: | (1) Center of Excellence in Genomics (CEG), International Crops Research Institute for the Semi-Arid Tropics (ICRISAT), Patancheru, Hyderabad, 502324, AP, India;(2) School of Life Sciences, University of Hyderabad, Hyderabad, 500046, AP, India;(3) University of Nottingham, University Park, Nottingham, NG7 2RD, UK;(4) College of Agriculture, Forestry and Life Sciences, Clemson University, Clemson, SC 29634, USA;(5) Center for Applied Genetic Technologies, University of Georgia, Athens, GA 30602, USA;(6) Genomics Towards Gene Discovery Sub Programme, Generation Challenge Programme, c/o CIMMYT, Int APDO Postal 6-641, 06600 Mexico, DF, Mexico; |
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| Abstract: | A set of 22 pearl millet inbred lines including the parents of eleven mapping populations, was screened with 627 markers including
100 pearl millet genomic SSRs (gSSRs), 60 pearl millet EST-SSRs (eSSRs), 410 intron sequence haplotypes (ISHs), and 57 exon
sequence haplotypes (ESHs). In all, 267 (59%) of the markers were informative for at least one of the 11 mapping populations,
which segregate for traits like drought and salinity tolerance; host plant resistance to downy mildew, rust and blast; fertility
restoration and sterility and maintenance of cytoplasmic male sterility etc. An average of 116 polymorphic markers was identified
per mapping population. The average PIC values and number of profiles (P) per polymorphic marker were: gSSRs (PIC = 0.62, P = 6.1), ISHs (PIC = 0.39, P = 2.6), eSSRs (PIC = 0.36, P = 3.1) and ESHs (PIC = 0.35, P = 3.1). A high correlation (r > 0.97, P < 0.05) was observed between the patterns of diversity exposed by the different marker systems. The polymorphic markers identified
are suitable for the de novo construction, or the supplementation of pearl millet linkage maps. The genetic relationships identified among the panel of
inbred lines may be useful in designing strategies to improve the use of available genetic variation in the context of pearl
millet breeding. |
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