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Effect of xylo-oligosaccharides from corn cobs autohydrolysis on the intestinal microbiota of piglets after weaning |
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| Authors: | P Moura S Marques L Alves JPB Freire LF Cunha MP Esteves |
| Institution: | aDepartamento de Biotecnologia, INETI, Estrada do Paço do Lumiar, 22, 1649–038 Lisboa, Portugal bUniversidade Técnicade Lisboa, Instituto Superior de Agronomia, Departamento de Produção Agrícola e Animal, Tapada da Ajuda, 1349-017 Lisboa, Portugal |
| Abstract: | The effect of xylo-oligosaccharides (XOS), a probiotic culture of Saccharomyces cerevisiae (SC) and the combined administration of XOS and SC on the piglet intestinal microbiota was investigated. Twenty four weaned piglets were fed during 4 weeks with one of the following diets: (BD) basal diet; (BD + XOS) basal diet supplemented with xylo-oligosaccharides (20 g kg? 1); (BD + SC) basal diet supplemented with S. cerevisiae (6 × 109 CFU kg? 1); and (BD + XOS + SC) basal diet supplemented with xylo-oligosaccharides and S. cerevisiae. Samples from the ileum, caecum and colon were collected and analysed for total anaerobes growing in XOS or glucose, xylose and arabinose (GXA) containing media. Specific primers were used to evaluate differences in Bifidobacterium and Lactobacillus populations and ERIC PCR to fingerprint the intestinal microbiota. The lowest number of culturable bacteria was obtained from the ileum of animals fed BD + XOS. The highest PCR titres with Lactobacillus group-specific primers in BD and BD + SC were obtained in the caecal contents. In XOS-supplemented diets the PCR titres were increased or maintained, from the caecum to the colon. Bifidobacteria were not detected in any samples using genus-specific primers. In the dendrogram from ERIC PCR, the piglets fed with XOS diets presented the highest similarity between samples and probiotic reference strains. |
| Keywords: | Xylo-oligosaccharide Pig Intestinal microbiota |
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