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Preliminary Studies on the Production of Artificial Seeds by Encapsutating Microtubers of Potato
作者姓名:TangWei  GuoZhongchen
作者单位:InstituteofBotany,ChineseAcadenyofSciences,Beijing100044,P.R.C
摘    要:The production of atificial seeds by encapsulating microtubers of potato included a series of proccesses.Initially,the micropropagation of adventitions buds and microtuber-inducing system should be established.Both high quatity microtuber and synchronizing microtuber which have the same size were necessary for producing health potato artificial seeds.In our eperiment,the optimum medium of adventitious shoot-inducing of potato Hutao,Kesi,and Favorita is DCR 1.0mg/kg MA 0.5mg/kg IBA 500mg/kg LH;the optium medium of microtuber-inducing of Hutao and Kesi is DCR 5.0mg/kg MA 200mg/kg Lh _8% sugar.and the optium medium of microtuber-inducing of Favorita is DCR 5.0 mg/kg BA 0.1mg/kg NAA 200 mg/kg LH 8% sugar,Microtubers of the same size were gained by selecting the symchronous microtubers.2.0% sodium alginate was sued to encapsulate the microtubers of potato to form the artificialo seeds.and the concentration of Ca Cl2 is 4% .Phytohormone NAA promoted the germination of potato artificial seeds.these artificial seeds have more than 90% germinating frequency in plant hormone-free DCR medium.

关 键 词:土豆  微块茎包封  人工种子生产  不定芽

Preliminary Studies on the Production of Artificial Seeds by Encapsutating Microtubers of Potato
Tang Wei,Guo Zhongchen,Gui Yaolin,Wu Meibao.Preliminary Studies on the Production of Artificial Seeds by Encapsutating Microtubers of Potato[J].Journal of Northeast Agricultural University,1996,3(2):156-159.
Authors:Tang Wei  Guo Zhongchen  Gui Yaolin  Wu Meibao
Abstract:The production of atificial seeds by encapsulating microtubers of potato included a series of processes. Initially, the micropropagation of adventitious buds and microtuber - inducing system should be established. Both high quality microtuber and synchronizing microtuber which have the same size were necessary for producing health potato artificial seeds. In our experiment, the optimum medium of adventitious shoot-inducing of potato Hutao, Kesi, and Favorita is DCR 1.0 mg / kg BA 0.5 mg / kg IBA 500 mg/ kg LH; the optium medium of microtuber- inducing of Hutao and Kesi is DCR 5.0 mg/ kg BA 200 mg/ kg Lh 8% sugar, and the optium medium of microtuber -inducing of Favorita is DCR 5.0 mg/ kg BA 0.1 mg / kg NAA 200 mg/ kg LH 8% sugar. Microtubers of the same size were gained by selecting the synchronous microtubers. 2.0% sodium alginate was used to encapsulate the microtubers of potato to form the artificial seeds, and the concentration of Ca C12 is 4%. Phytohormone NAA promoted the germination of potato artificial seeds. These artificial seeds have more than 90% germinating frequency in plant hormone-free DCR medium.
Keywords:Solanum tuberosum  adventitious shoot  microtuber  artificialseed  phytohormones
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