| mRNA差异显示分离棉花抗旱耐盐基因的相关cDNA片段 |
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| 引用本文： | 聂新辉,曲延英,尤春源,陈全家.mRNA差异显示分离棉花抗旱耐盐基因的相关cDNA片段[J].新疆农业大学学报,2007,30(4):72-75. |
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| 作者姓名： | 聂新辉 曲延英 尤春源 陈全家 |
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| 作者单位： | 新疆农业大学,农学院,乌鲁木齐,830052 |
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| 基金项目： | 新疆高等学校科研项目;新疆维吾尔自治区高新技术 |
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| 摘 要： | 采用mRNA差异显示(DDRT-PCR)技术,对抗旱、耐盐棉花品种(品系)分别进行25%PEG6000和1.3%NaCl胁迫诱导,利用优化的蛋白酶K法分别提取胁迫与非胁迫棉花叶片中的总RNA,以此为模板,选用12个锚定引物和20个随机引物的240个组合进行RT-PCR,产物经6%变性聚丙烯酰胺凝胶电泳后银染,共获得88条差异条带,二次PCR扩增,选择出41条表达稳定的差异条带。
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| 关 键 词： | DDRT-PCR技术 抗旱 耐盐 蛋白酶K法 聚丙烯酰胺凝胶电泳 |
| 文章编号： | 1007-8614（2007）04-0072-04 |
| 修稿时间： | 2007-05-10 |
Isolation of cDNA Fragment Related to Cotton Resistance to Drought and Salt Genes by mRNA Differential Display |
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| NIE Xing-hui,QU Yan-ying,YOU Chun-yuan,CHEN Quan-jia.Isolation of cDNA Fragment Related to Cotton Resistance to Drought and Salt Genes by mRNA Differential Display[J].Journal of Xinjiang Agricultural University,2007,30(4):72-75. |
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| Authors: | NIE Xing-hui QU Yan-ying YOU Chun-yuan CHEN Quan-jia |
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| Institution: | College of Agronomy, Xinjiang Agricultural University, Urumqi, 830052 |
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| Abstract: | Using mRNA differential display(DDRT-PCR),the cotton variety of resistance to drought and salt is treated separately by 25%PEG6000 and 1.3% NaCl.The total RNA of the treated and non-treated cotton leaves are drawed separately by using the way of improved proteinase-K pathway.The 240 combinations which are established by 12 anchored primers and 20 arbitrary primers are conducted by RT-PCR,the products are applied by the method of silver-staining with 6% PAGE.88 different displayed fragments are obtained,and 41 of which are picked up by the second PCR. |
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| Keywords: | DDRT-PCR technique resistance to drought resistance to salt proteinase-K pathway Polypropylene gel electrophoresis |
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