| 水稻细菌性条斑病非寄主抗性基因Rxo1的双右边界双元载体的构建 |
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| 引用本文： | 许美容,夏志辉,翟文学,徐建龙,周永力,黎志康.水稻细菌性条斑病非寄主抗性基因Rxo1的双右边界双元载体的构建[J].中国水稻科学,2008,22(2):208. |
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| 作者姓名： | 许美容 夏志辉 翟文学 徐建龙 周永力 黎志康 |
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| 作者单位： | 1.中国农业科学院 作物科学研究所/农作物基因资源与基因改良国家重大科学工程， 北京 100081;2.中国科学院 遗传与发育生物学研究所， 北京100101； *通讯联系人， E-mail: zhouyl@caas.net.cn |
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| 摘 要： | Rxo1 是从玉米中克隆的水稻细菌性条斑病非寄主抗性基因。采用ScaⅠ和NgoMⅣ酶切携带Rxo1 基因的载体pCAMBIA1305 1，HpaⅠ和XmaⅠ酶切双右边界双元载体pMNDRBBin6，通过平端和黏性末端连接目的基因和载体，得到了重组载体pMNDRBBin6 Rxo1。 PCR和限制性内切酶酶切以及序列测定证实Rxo1 整合到了双右边界载体pMNDRBBin6中。该载体为利用转基因技术获得无选择标记抗细菌性条斑病水稻育种材料提供了条件。
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| 关 键 词： | Rxo1 基因 双右边界双元载体 水稻 细菌性条斑病 |
Construction of Double Right-Border Binary Vector Carrying Non-Host Gene Rxo1 Resistant to Rice Bacterial Leaf Streak |
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| XU Mei-rong,XIA Zhi-hui,ZHAI Wen-xue,XU Jian-long,ZHOU Yong-li,LI Zhi-kang.Construction of Double Right-Border Binary Vector Carrying Non-Host Gene Rxo1 Resistant to Rice Bacterial Leaf Streak[J].Chinese Journal of Rice Science,2008,22(2):208. |
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| Authors: | XU Mei-rong XIA Zhi-hui ZHAI Wen-xue XU Jian-long ZHOU Yong-li LI Zhi-kang |
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| Abstract: | Rxo1 cloned from maize was a non-host gene resistant to bacterial leaf streak of rice.pCAMBIA1305-1 withRxo1 was digested withScaⅠandNgoM Ⅳ and the double right-border binary vector pMNDRBBin6 was digested withHpaⅠandXmaⅠ.pMNDRBBin6 carrying the geneRxo1 was acquired by ligation of blunt-end and cohesive end.The results of PCR,restriction digestion and sequencing showed that theRxo1 gene has been cloned into pMNDRBBin6.This double right-border binary vector,being named as pMNDRBBin6-Rxo1,would play a role in breeding marker-free plants resistant to rice bacterial leaf streak by genetic transformation. |
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| Keywords: | Rxo1 gene double right-border binary vector rice bacterial leaf streak marker-free |
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