Gene expression analysis of peroxisome proliferators- and phenytoin-induced hepatotoxicity using cDNA microarray |
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| Authors: | Jung Jin-Wook Park Joon-Suk Hwang Jae-Woong Kang Kyung-Sun Lee Yong-Soon Song Bog-Soo Lee Gyoung-Jae Yeo Chan-Dong Kang Jong-Soo Lee Wan-Seon Jeon Ki-Seon Um Chan-Hwi Kim Yang-Suk Oh Moon-Ju Youn Jong-Pil Li Peng Park Jung-Eun Hwang Seung Yong |
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| Institution: | Department of Biochemistry and Molecular Biology, Hanyang University & Genocheck Co., Ltd., South Korea. |
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| Abstract: | The recent DNA microarray technology enables us to understand a large number of gene expression profiling. The technology has potential possibility to comprehend mechanism of multiple genes were related to compounds which have toxicity in biological system. So, the toxicogenomics through this technology may be very powerful for understanding the effect of unknown toxic mechanisms in biological system. We have studied that the effect of compounds related to hepatotoxin in vivo system using DNA microarray and classified chemicals which have been well characterized. We have studied three compounds; 2 peroxisome proliferators: Clofibrate (ethyl-p-chlorophenoxyisobutyrate), gemfibrozil (5-22,5-dimethyl-phenoxy]2-2-dimethyl-pentanonic), and an antiepileptic drug: phenytoin (5,5-diphenylhydantoin). Male Sprague-Dawely VAF(+) albino rats of 5-6 weeks old were treated with each compound for 24 hr and 2 weeks. 4.8 K cDNA microarray in house has been used for gene expression profiling. We found that the clustering of gene expression had similarity like as the toxic phenotype of compounds. |
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