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Soil solid phases effects on the proteomic analysis of Cupriavidus metallidurans CH34 |
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| Authors: | Laura Giagnoni Francesca Magherini Loretta Landi Safiyh Taghavi Daniel van der Lelie Michele Puglia Laura Bianchi Luca Bini Paolo Nannipieri Giancarlo Renella Alessandra Modesti |
| Institution: | 1. Department of Plant, Soil and Environmental Sciences, University of Florence, Piazzale delle Cascine, 28, Florence, Italy 2. Department of Biochemical Sciences, University of Florence, Florence, Italy 3. Biology Department, Brookhaven National Laboratory, Upton, NY, USA 4. RTI International, Research Triangle Park, NC, USA 5. Department of Molecular Biology, University of Siena, Siena, Italy |
| Abstract: | Cupriavidus metallidurans CH34 is a completely sequenced soil-borne beta-proteobacterium with known genome and proteome. Comparative 2-D electrophoresis and protein mass spectrometry were used to compare the proteome of C. metallidurans CH34 from liquid culture and after incubation for 1, 3, and 12?days in microcosms containing quartz sand, kaolinite, montmorillonite, or an artificial soil. Results showed that proteome from liquid culture was similar to CH34 proteins extracted from sand and kaolinite, whereas the proteins extracted from artificial soil differed significantly and no proteins were detected from C. metallidurans CH34 incubated in the montmorillonite microcosms. Protein recovery decreased on prolonging incubation time in all microcosms. Mass spectrometry identification showed that the trend of lower recovery upon incubation time was independent on the putative function of protein. These results suggest that the soil solid phase influences the protein recovery and soil proteomic analysis and that distinction between protein recovery and protein expression in soil will be a challenging for soil proteomic researchers. |
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