| HPLC法测定大豆苷元的平衡溶解度和表观油水分配系数 |
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| 引用本文： | 于秀珍,何珺,钱一鑫,李洪庆.HPLC法测定大豆苷元的平衡溶解度和表观油水分配系数[J].安徽农业科学,2009,37(10):4367-4368. |
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| 作者姓名： | 于秀珍 何珺 钱一鑫 李洪庆 |
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| 作者单位： | 贵州大学贵州省生化工程中心,贵州贵阳,550025;贵州大学贵州省生化工程中心,贵州贵阳,550025;贵州大学贵州省生化工程中心,贵州贵阳,550025;贵州大学贵州省生化工程中心,贵州贵阳,550025 |
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| 基金项目： | 贵州省科技厅工业攻关项目(黔科合GY字(2007)3026) |
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| 摘 要： | 目的]测定大豆苷元在各种溶剂中的平衡溶解度以及在正辛醇-水和正辛醇-缓冲液体系中的表观油水分配系数。方法]采用HPLC法测定大豆苷元的浓度,采用摇瓶法测定大豆苷元的表观油水分配系数。结果]25℃大豆苷元在水中的平衡溶解度为0.89mg/L。在碱性磷酸盐缓冲液中的平衡溶解度较酸性磷酸盐缓冲液中高;大豆苷元的表观油水分配系数Papp为191.3（1gPapp=2.28）。结论]大豆苷元的水溶性较差,提高其口服制剂的溶出度有利于提高其生物利用度。
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| 关 键 词： | 大豆苷元 平衡溶解度 表观油水分配系数 高效液相色谱法 |
Determination of Equilibrium Solubility and Apparent Oil/Water Partition Coefficient of Daidzein By HPLC |
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| YU Xiu-zhen et al.Determination of Equilibrium Solubility and Apparent Oil/Water Partition Coefficient of Daidzein By HPLC[J].Journal of Anhui Agricultural Sciences,2009,37(10):4367-4368. |
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| Authors: | YU Xiu-zhen |
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| Institution: | YU Xiu-zhen et al(Research Center of Biochemistry Engineering of Guizhou Province,Guizhou University,Guiyang,Guizhou 550025) |
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| Abstract: | Objective] The study aims to determine the equilibrium solubility of Daidzein in various solvents and its apparent oil/water partition coefficients for the n-octanol-water/ buffer solution systems.Method] The concentration of daidzein is determined by HPLC,apparent oil/water partition coefficient is determined by shaking flask method.Result] The equilibrium solubility of daidzein in water is 0.89 mg/ L at 25 ℃ and higher in alkaline phosphate buffer than in others.Papp of Daidzein is 191.3(1gPapp=2.28).Conclusion] Water solubility of Daidzein is bad,increasing the dissolution rate of Daidzein may have the possibility to enhance its bioavailability. |
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| Keywords: | Daidzein Equilibrium solubility Apparent Oil/Water partition coefficient HPLC |
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