| 灵芝胞内多糖高产菌株G7深层发酵工艺的研究 |
| |
| 引用本文： | 余素萍,张劲松,杨焱,贾薇,潘迎捷.灵芝胞内多糖高产菌株G7深层发酵工艺的研究[J].中国食用菌,2004,23(6):36-39. |
| |
| 作者姓名： | 余素萍 张劲松 杨焱 贾薇 潘迎捷 |
| |
| 作者单位： | 1. 南京农业大学微生物学系,南京,210095;上海农科院食用菌研究所,上海市农科院天然药用资源研究与开发中心,上海市农业遗传育种重点开放实验室,农业部食用菌遗传育种重点开放实验室,上海,201106
2. 上海农科院食用菌研究所,上海市农科院天然药用资源研究与开发中心,上海市农业遗传育种重点开放实验室,农业部食用菌遗传育种重点开放实验室,上海,201106 |
| |
| 基金项目： | 本项目由上海市科委项目支持 |
| |
| 摘 要： | 通过摇瓶培养实验从 2 2个广泛栽培的灵芝菌株中筛选出了胞内多糖产量最高的菌株 ,并以胞内多糖产量为指标通过单因子及正交实验优化了该菌株深层发酵所需的营养因子及非营养因子条件 ,包括碳源、氮源、初始pH、接种量、装液量、培养时间等。研究发现G7的最佳培养条件及目标产物不同 ,所需的培养时间也不同。G7在对数期内大量形成胞内多糖 ,在 6 0h胞内多糖产量最高达 5 33mg/mL培养液
|
| 关 键 词： | 灵芝 胞内多糖 营养因子 非营养因子 |
| 文章编号： | 1003-8310(2004)06-0036-04 |
| 修稿时间： | 2004年4月7日 |
Investigation of Submerged Fermentation Parameters of Ganoderma Strain G7 of High Yield Intracellular Polysaccharide |
| |
| YU Su-ping.Investigation of Submerged Fermentation Parameters of Ganoderma Strain G7 of High Yield Intracellular Polysaccharide[J].Edible Fungi of China,2004,23(6):36-39. |
| |
| Authors: | YU Su-ping |
| |
| Institution: | YU Su-ping~ |
| |
| Abstract: | The strain G7 was screened out from 22 Ganoderma strains collected from various regions according to the yield of intracellular polysaccharide.The optimum fermentation conditions were studied by single factor experiment and orthogonal test.The optimum parameters of carbon source,nitrogen source,initial hither medium volume in the flask and cell density were obtained.In addition,the metabolic curves of intracellular polysaccharide,extracellular polysaccharide,biomass and the pH change of medium were determined.It was found that the optimal fermentation time was depended on the objective product.Most of the intracellular polysaccharide was produced within the former stage of fermentation.The yield of intracellular polysaccharide was up to 0.533 g/100 mL medium at the 60th hour. |
| |
| Keywords: | Ganoderma Intracellular polysaccharide Nutritional factors Non-nutritional factors |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
|
