| 几种氨基酸前体物对红豆杉愈伤组织的生长和紫杉醇含量的影响 |
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| 引用本文： | 翟雪霞,李友勇.几种氨基酸前体物对红豆杉愈伤组织的生长和紫杉醇含量的影响[J].湖北农业科学,2009,48(10). |
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| 作者姓名： | 翟雪霞 李友勇 |
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| 作者单位： | 1. 河南师范大学生命科学学院,河南,新乡,453007;河南科技学院生命科技学院,河南,新乡,453003
2. 河南科技学院生命科技学院,河南,新乡,453003 |
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| 摘 要： | 通过在培养基中加入紫杉醇合成的前体物质,提高愈伤组织紫杉醇含量和细胞生物量.在培养基中添加甘氨酸、L-丝氨酸、L-苯丙氨酸和D-苯丙氨酸,培养40 d后,测定愈伤组织紫杉醇含量和细胞生物量.分析得到甘氨酸、丝氨酸、L-苯丙氨酸能提高细胞紫杉醇的合成能力,但对愈伤组织的生长有抑制作用.D-苯丙氨酸对愈伤组织的生长有显著的促进作用,也显著提高紫杉醇合成能力,愈伤组织中紫杉醇含量高达0.224%.
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| 关 键 词： | 红豆杉 愈伤组织培养 紫杉醇 D-苯丙氨酸 |
Effect of Amino Acid Precursors on Callus Growth and Taxol Content |
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| ZHAI Xue-xia,LI You-yong.Effect of Amino Acid Precursors on Callus Growth and Taxol Content[J].Hubei Agricultural Sciences,2009,48(10). |
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| Authors: | ZHAI Xue-xia LI You-yong |
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| Abstract: | Objective] For increasing growth production and taxol content of callus in cell cultures of Chinese yew by addition precursors. Methods] Addition glycine,L-serine,L-phenylalanine and D-phenylalanine in the medium. After 40 days the callus production and the taxol conten was determinated. Results] Glycine,L-serine and L-phenylalanine could improve the ability of synthesis taxol,but had inhibition to cells growth. However,D-phenylalanine could promote the cells growth and improve taxol synthesis. The taxol content was as high as 0.224%. |
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| Keywords: | Chinese yew callus culture taxol D-phenylalanine |
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