| 小麦—簇毛麦6VS／6AL易位系叶片cDNA文库构建及鉴定 |
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| 引用本文： | 牛吉山,于玲,陈佩度,刘大钧.小麦—簇毛麦6VS／6AL易位系叶片cDNA文库构建及鉴定[J].南京农业大学学报,2001,24(1):5-8. |
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| 作者姓名： | 牛吉山 于玲 陈佩度 刘大钧 |
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| 作者单位： | 南京农业大学农业部 |
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| 基金项目： | 86 3计划资助项目! (Z 17 0 4 0 1) |
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| 摘 要： | 用表达型质粒载体p^SPORT1构建了经白粉菌（Erysiphe graminis)诱导48h和未经诱导的小麦－簇毛麦6VS／6AL易位系（Triticum aestivum-Haynaldia villosa translocation lin)叶片cDNA库各一个，库宿主菌为E.coli DH10B，非诱导库包含约50万个重组cDNA克隆，平均插入征段为1．25kb，主要分布在400bp-2.2kb。诱导民含约30万个重组cDNA克隆，平均插入片段1．2kb。用克隆的病程相关基因（pathogenesis related gene)，＝－小麦类甜蛋白基因pWIR作探针，进行杂交筛库，杂交信号明显，重复性好。
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| 关 键 词： | 小麦－簇毛麦6VS／6AL易位系 cDNA文库 白粉病 基因克隆 抗病基因 鉴定 |
| 文章编号： | 1000-2030(2001)01-0005-04 |
| 修稿时间： | 2000年9月6日 |
cDNA library construction and identification of Triticum aestivum Haynaldia villosa 6VS/6AL translocation line |
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| Niu Jishan,Yu Lin,Chen Peidu,Liu Dajun.cDNA library construction and identification of Triticum aestivum Haynaldia villosa 6VS/6AL translocation line[J].Journal of Nanjing Agricultural University,2001,24(1):5-8. |
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| Authors: | Niu Jishan Yu Lin Chen Peidu Liu Dajun |
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| Institution: | Niu Jishan,Yu Ling,Chen Peidu * and Liu Dajun |
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| Abstract: | Two cDNA libraries of Triticum aestivum-Haynald ia villosa 6VS／6AL translocation line were constructed using mRNA from leaves of its plants induced and non-induced with Erysiphe graminis.An expression pla smid pSPORT 1 introduced into E.coli DH10B was used as cloning vector. The cDNA library of pathogen induced translocation line comprised of about 300 0 00 recombinant cDNA clones with average insert size of 1.2 kb,and that of non -induced translocation line consisted of aproximately 500 000 recombinant cDNA clones with average insert size of 1.25 kb,ranging ba sically from 0.4 to 2.2 kb.The library was successfully screened with a wheat thaumatin-like protein gene pWIR as the probe. |
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| Keywords: | VS/6AL translocation line cDNA library Erysiphe graminis induce |
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