| T-DNA插入麝香百合的研究(英文) |
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| 作者单位： | 中国热带农业科学院生物技术研究所 海南海口571101 |
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| 摘 要： | Objective] The aim of this study is to obtain transgenic Lilium longiflorum Thumb. Method] A two-step method of explant and the T-DNA integration technique were employed to transform Lilium longiflorum via Agrobacterium mediated method. Result] The best infection effect appeared under the OD600 value of Agrobacterium within 0.6-0.8,the addition of 250 mg/L AS could increase the transformation efficiency. The optimal concentration of G418 for screening is 50 mg/L. Some putative transgenic plants of Lilium longiflorum with resistance to G418 showed positive in PCR,preliminarily proving that T-DNA gene had integrated into the genome of lily. Conclusion] The study may lay a foundation for breeding excellent lily varieties through T-DNA integration technique.
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Study on the Integration of T-DNA into Lilium longiflorum |
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| Abstract: | Objective] The aim of this study is to obtain transgenic Lilium longiflorum Thumb. Method] A two-step method of explant and the T-DNA integration technique were employed to transform Lilium longiflorum via Agrobacterium mediated method. Result] The best infection effect appeared under the OD600 value of Agrobacterium within 0.6-0.8,the addition of 250 mg/L AS could increase the transformation efficiency. The optimal concentration of G418 for screening is 50 mg/L. Some putative transgenic plants of Lilium longiflorum with resistance to G418 showed positive in PCR,preliminarily proving that T-DNA gene had integrated into the genome of lily. Conclusion] The study may lay a foundation for breeding excellent lily varieties through T-DNA integration technique. |
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| Keywords: | Lilium longiflorum Agrobacterium-mediated transformation T-DNA Insertion mutation |
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