| 低能铁离子注入烟草叶片的过氧化物酶同工酶和全蛋白分析 |
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| 引用本文： | 焦浈,秦广雍,霍裕平.低能铁离子注入烟草叶片的过氧化物酶同工酶和全蛋白分析[J].河南农业科学,2004(6):23-25. |
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| 作者姓名： | 焦浈 秦广雍 霍裕平 |
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| 作者单位： | 郑州大学离子束生物工程省重点实验室,河南,郑州,450052;郑州大学离子束生物工程省重点实验室,河南,郑州,450052;郑州大学离子束生物工程省重点实验室,河南,郑州,450052 |
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| 基金项目： | 国家“十五”科技攻关项目 ( 2 0 0 1BA3 0 2B -0 3 ) |
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| 摘 要： | 低能铁离子注入烟草叶片后 ,过氧化物酶 (POD)同工酶表达量和谱带发生了明显的变化。叶片中诱导出一条活性很强的Rf'1 同工酶谱带 ,缺失Rf5～Rf73条酶带。全蛋白的变化主要发生在蛋白表达量的差异上 ,与POD同工酶的变化具有相关性。POD同工酶谱的变化反映了注入造成的损伤在修复过程中细胞的生理生化变化 ,为进一步揭示低能离子注入的作用机理提供了基础。
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| 关 键 词： | 低能铁离子注入 烟草 过氧化物酶(POD) 同工酶 全蛋白 |
| 文章编号： | 1004-3268(2004)06-0023-03 |
| 修稿时间： | 2004年3月29日 |
Analysis on Peroxidase Isozyme and Protein in Tobacco Leaves Implanted by Low Energy Iron Ion |
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| JIAO Zhen,QIN Guang-yong,HUO Yu-ping.Analysis on Peroxidase Isozyme and Protein in Tobacco Leaves Implanted by Low Energy Iron Ion[J].Journal of Henan Agricultural Sciences,2004(6):23-25. |
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| Authors: | JIAO Zhen QIN Guang-yong HUO Yu-ping |
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| Abstract: | Peroxidese (POD) isozyme content and zymogram changed remarkably during the sterile culture after low energy iron ion-beam was implanted into tobacco leaves. Rf ' 1 with strong activity was induced in the leaves, while Rf 5-Rf 7 were absent after implantation treatment. The change of protein was mainly about the difference of contents,which was relatively with change of POD isozyme. POD zymogram reflected chemophysiology change of cell restored from the implanted damage. Results above provides the base for further study on mechanism of low energy ion-beam implantation. |
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| Keywords: | Low energy iron ion-beam implant Tobacco Peroxidase Isozyme Protein |
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