| 五种杨树叶绿体DNA的提取及RFLP分析 |
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| 引用本文： | 卢孟柱,卞祖娴.五种杨树叶绿体DNA的提取及RFLP分析[J].林业科学研究,1992,5(4):465-468. |
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| 作者姓名： | 卢孟柱 卞祖娴 |
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| 作者单位： | 中国林业科学研究院林业研究所
(卢孟柱)，中国林业科学研究院林业研究所(卞祖娴) |
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| 摘 要： |
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| 关 键 词： | 杨树 叶绿体 DNA 提取 RFLP |
| 收稿时间： | 1992/2/21 0:00:00 |
Studies on the Isolation and RFLP Analysis of Poplar Chloroplast DNA |
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| Lu Mengzhu and Bian Zuxian.Studies on the Isolation and RFLP Analysis of Poplar Chloroplast DNA[J].Forest Research,1992,5(4):465-468. |
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| Authors: | Lu Mengzhu and Bian Zuxian |
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| Institution: | The Research Institute of Forestry CAF |
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| Abstract: | Chloroplast DNA (cpDNA) were prepared from five Poplar samples (two hybrids of Populus deltoides Bartr. CL. 'Lux' x P. deltoides Bartr. CL. 'Harvard', two natural hybrids of P. deltoides and one sample of P. laurifolia) and analysed with five restriction enzymes. The results show that although the cpDNA diversity between Sect. Tacamahaca Spach. and Sect. Aigeiros Duby is low, they can be distinguished by using the restriction fragment length polymorphisms (RFLP) generated by EcoR I and BamH I . The RFLP generated by BamH I can also be used to distinguish the two natural hybrids of P. deltodes. So the analysis of Poplar cpDNA RFLP provides a molecular biological method for Poplar classification, cross breeding program and phylogenetic studies. |
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| Keywords: | Poplar chloroplast DNA restriction fragment length polymorphisms (RFLP) |
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