| 海南龙血树离体快繁的研究 |
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| 引用本文： | 陈梅,莫饶.海南龙血树离体快繁的研究[J].安徽农业科学,2008,36(3):968-968,1068. |
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| 作者姓名： | 陈梅 莫饶 |
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| 作者单位： | 华南热带农业大学农学院,海南儋州,571737;华南热带农业大学农学院,海南儋州,571737 |
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| 摘 要： | 目的]加强龙血树组织培养的再生体系,为其迅速繁殖及大规模工厂化育苗提供依据。方法]以龙血树的幼嫩茎段为外植体,用9种6-BA、NAA不同浓度配比的诱导培养基和NAA不同浓度的生根培养基进行对比试验,研究海南龙血树的组织培养与快速繁殖。结果]龙血树幼嫩茎段的诱导和生长与6-BA、NAA有关系,两者的配比直接影响茎段的再生频率。6-BA浓度对愈伤组织的形成影响极为显著,NAA的影响不是很明显。MS+0.3 mg/L NAA的生根时间最短,15 d左右生根,根数多,根系良好,移栽成活率高达95%。结论]龙血树幼嫩茎段的诱导和不定芽增殖的最适培养基为MS+6.0 mg/L 6-BA+0.5 mg/L NAA,增殖系数达10以上,最适宜的生根培养基为MS+0.3 mg/L NAA。
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| 关 键 词： | 海南龙血树 组织培养 快速繁殖 |
| 文章编号： | 0517-6611（2008）03-00968-01 |
| 收稿时间： | 2007-09-28 |
| 修稿时间： | 2007年9月28日 |
Study on Rapid Propagation in vitro of Dracaena cambodiana |
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| CHIEN Mei et al.Study on Rapid Propagation in vitro of Dracaena cambodiana[J].Journal of Anhui Agricultural Sciences,2008,36(3):968-968,1068. |
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| Authors: | CHIEN Mei |
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| Abstract: | Objective] The research aimed to strengthen the tissue culture regeneration system of Dracaena cambodiana and provide basis for its rapid propagation and industrial seedling on a large scale.Method] With tender stem segments of Dracaena cambodiana as explants,comparative test was conducted on 9 kinds of induction medium with different concentrations of 6-BA and NAA,and rooting medium with different concentrations of NAA to study the tissue culture and rapid propagation of Dracaena cambodiana.Result] The induction and growth of tender stem segment of D.cambodiana had relation with 6-BA and NAA and the matching ratio of the two directly affected the regeneration frequency of stem segment.The effect of 6-BA concentration on the formation of callus was extremely significantly and the effect of NAA was not obvious.The rooting time of MS NAA 0.3 mg/L was shortest,about 15 d,with much roots and better root system and the transplanting survival rate reached 95%.Conclusion] The optimum medium for the induction of tender stem segment and the propagation of adventitious buds in Dracaena cambodiana was MS 6-BA 6.0 mg/L NAA 0.5 mg/L and its propagation coefficient reached over 10.The optimum rooting medium was MS NAA 0.3 mg/L. |
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| Keywords: | Dracaena cambodiana Tissue culture Rapid propagation |
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